Winkler P, Ellinger S, Boetzer A M, Arendt B M, Berthold H K, Rockstroh J K, Spengler U, Goerlich R
School of Medicine, Department of Hemostasis and Transfusion Medicine, University of Duesseldorf, Duesseldorf, Germany.
Eur J Clin Nutr. 2004 Feb;58(2):317-25. doi: 10.1038/sj.ejcn.1601784.
We investigated whether ingestion of polyphenols from fruit juices or a fruit-vegetable-concentrate affects lymphocyte proliferation and apoptosis in human immunodeficiency virus (HIV)-seropositive (HIV(+)) and HIV-seronegative (HIV(-)) subjects.
Randomized, prospective pilot intervention study.
University of Bonn, Department of General Internal Medicine.
A total of 23 HIV(+) subjects from the HIV outpatient clinic, 18 HIV(-) controls.
Subjects ingested either 1 l of fruit juice or 30 ml of fruit-vegetable-concentrate daily for 16 weeks in addition to their regular diet. Lymphocyte proliferation and apoptosis were investigated in peripheral blood mononuclear cells at baseline, during 16-weeks of intervention, and after a 6-week washout. Proliferation was assessed by (3)H-thymidine incorporation and apoptosis by nuclear content as measured by flow cytometry.
Supplementation of fruit juices increased phytohemagglutinin-induced lymphocyte proliferation (mitotic index) in HIV(+) patients from 18+/-16 to 40+/-34 (P=0.004) and in healthy controls from 27+/-16 to 51+/-21 (P=0.016). Apoptosis was not affected in HIV(+) patients, but rose in healthy controls from 9+/-10 to 34+/-11 (apoptotic index; P=0.001). Intervention with concentrate did not significantly alter proliferation and apoptosis in HIV(+) and HIV(-) subjects.
Even though apoptosis did not change in HIV(+) subjects, ingestion of polyphenol-rich fruit juices might be favorable to HIV(+) patients due to enhanced proliferation, which could restore disturbances in T-cell homeostasis. In healthy controls, increased lymphocyte proliferation during juice consumption was counterbalanced by increased apoptosis.
我们研究了饮用果汁或果蔬浓缩液中的多酚类物质是否会影响人类免疫缺陷病毒（HIV）血清阳性（HIV(+)）和HIV血清阴性（HIV(-)）受试者的淋巴细胞增殖和凋亡。
随机、前瞻性试点干预研究。
波恩大学，普通内科。
来自HIV门诊的23名HIV(+)受试者，18名HIV(-)对照者。
受试者除常规饮食外，每天饮用1升果汁或30毫升果蔬浓缩液，持续16周。在基线、16周干预期间以及6周洗脱期后，对外周血单核细胞中的淋巴细胞增殖和凋亡进行研究。通过³H-胸腺嘧啶核苷掺入法评估增殖，通过流式细胞术测量核含量评估凋亡。
补充果汁可使HIV(+)患者中植物血凝素诱导的淋巴细胞增殖（有丝分裂指数）从18±16增至40±34（P = 0.004），健康对照者从27±16增至51±21（P = 0.016）。HIV(+)患者的凋亡未受影响，但健康对照者的凋亡从9±10增至34±11（凋亡指数；P = 0.001）。浓缩液干预未显著改变HIV(+)和HIV(-)受试者的增殖和凋亡。
尽管HIV(+)受试者的凋亡没有变化，但摄入富含多酚的果汁可能对HIV(+)患者有益，因为增殖增强，这可能恢复T细胞稳态的紊乱。在健康对照者中，饮用果汁期间淋巴细胞增殖增加被凋亡增加所抵消。
