使用四种颜色的量子点荧光试剂进行多重毒素分析。

Multiplexed toxin analysis using four colors of quantum dot fluororeagents.

作者信息

Goldman Ellen R, Clapp Aaron R, Anderson George P, Uyeda H Tetsuo, Mauro J Matthew, Medintz Igor L, Mattoussi Hedi

机构信息

Center for Bio/Molecular Science and Engineering and Division of Optical Sciences, U.S. Naval Research Laboratory, Washington, D.C. 20375, USA.

出版信息

Anal Chem. 2004 Feb 1;76(3):684-8. doi: 10.1021/ac035083r.

DOI:10.1021/ac035083r

PMID:14750863

Abstract

Quantum dots (QDs) have the potential to simplify the performance of multiplexed analysis. In this work, we prepared bioinorganic conjugates made with highly luminescent semiconductor nanocrystals (CdSe-ZnS core-shell QDs) and antibodies to perform multiplexed fluoroimmunoassays. Sandwich immunoassays for the detection of cholera toxin, ricin, shiga-like toxin 1, and staphylococcal enterotoxin B were performed simultaneously in single wells of a microtiter plate. Initially the assay performance for the detection of each toxin was examined. We then demonstrated the simultaneous detection of the four toxins from a single sample probed with a mixture of all four QD-antibody reagents. Using a simple linear equation-based algorithm, it was possible to deconvolute the signal from mixed toxin samples, which allowed quantitation of all four toxins simultaneously.

摘要

量子点（QDs）有潜力简化多重分析的操作。在这项工作中，我们制备了由高发光半导体纳米晶体（CdSe-ZnS核壳量子点）和抗体组成的生物无机共轭物，以进行多重荧光免疫测定。在微量滴定板的单个孔中同时进行用于检测霍乱毒素、蓖麻毒素、志贺样毒素1和葡萄球菌肠毒素B的夹心免疫测定。最初检查了每种毒素检测的测定性能。然后，我们展示了用所有四种量子点-抗体制剂的混合物探测单个样品时对四种毒素的同时检测。使用基于简单线性方程的算法，可以对混合毒素样品的信号进行解卷积，从而能够同时对所有四种毒素进行定量。