Aksoy Umit, Ustun Sebnem, Dagci Hande, Yazar Süleyman
Department of Parasitology, Medical Faculty, Dokuz Eylül University, Znciralti, Zzmir, Turkey.
World J Gastroenterol. 2004 Feb 1;10(3):449-51. doi: 10.3748/wjg.v10.i3.449.
The effects of cobalt, copper, cadmium and barium ions on the cysts of Entamoeba histolytica (E. histolytica), an amebic dysentery agent, cultured in Robinson medium were investigated.
E. histolytica cysts and trophozoites isolated from a patient with amebiasis were cultivated in the medium, incubated at 37 degrees for a period of 4 days and 40 x 10(4)/ml amebic cysts were then transferred to a fresh medium. At the second stage, 0.05, 0.1 and 0.2 mM of selected metal ions were added to the medium, and the effects of these ions on parasitic reproduction compared with the control group were observed.
It was determined that the number of living parasites in all the groups containing metal ions decreased significantly starting from 30 minutes (P<0.01). CuCl2 showed the highest lethal effect on E. histolytica cysts, whereas the lowest lethal effect was observed with CoCl2. It was also seen that the number of living cells was decreased as the ion concentration and exposure time were increased, and that there were no living parasites in the medium at the end of 24 h (P<0.01).
It may be stated that the effect of ever-increasing contamination of the environment with metal waste materials on parasites should be investigated further.
研究钴、铜、镉和钡离子对在罗宾逊培养基中培养的溶组织内阿米巴（一种阿米巴痢疾病原体）包囊的影响。
从一名阿米巴病患者分离出的溶组织内阿米巴包囊和滋养体在该培养基中培养，于37℃孵育4天，然后将40×10⁴/ml的阿米巴包囊转移至新鲜培养基中。在第二阶段，向培养基中添加0.05、0.1和0.2 mM的选定金属离子，并观察这些离子与对照组相比对寄生虫繁殖的影响。
确定所有含金属离子组中活寄生虫数量从30分钟起显著减少（P<0.01）。CuCl₂对溶组织内阿米巴包囊显示出最高的致死作用，而CoCl₂的致死作用最低。还观察到随着离子浓度和暴露时间增加，活细胞数量减少，并且在24小时结束时培养基中没有活寄生虫（P<0.01）。
可以说，环境中金属废料污染不断增加对寄生虫的影响应进一步研究。
