Song Hwa-Young, Jeong Da-Eun, Lee Mina
College of Pharmacy and Research Institute of Life and Pharmaceutical Sciences, Sunchon National University, 255 Jungangno, Suncheon 57922, Korea.
Institute of Jinan Red Ginseng, 41 Hongsamhanbangno, Jinan-Gun 55442, Korea.
Plants (Basel). 2021 Jul 28;10(8):1545. doi: 10.3390/plants10081545.
The aim of this study was to identify the optimal extraction conditions for leaves of var. . Inhibitory effects of various extracts on NO production were compared. Antioxidant evaluations for total phenol and flavonoid contents were carried out using various extracts of var. leaves obtained under optimal extraction conditions that showed the greatest effect on NO production. The optimal method for extracting var. leaves resulted in an extract named OP OFLE. OP OFLE showed DPPH and ABTS radical scavenging activities in a concentration-dependent manner. Phillyrin (PH) was isolated as a major compound from OP OFLE by HPLC/DAD analysis. OP OFLE and PH reduced inducible nitric oxide (iNOS) and cyclooxygenase (COX)-2 protein expression and downregulated proinflammatory cytokines such as interleukin (IL)-1β, IL-6, IL-8, and tumor necrosis factor (TNF)-α in LPS-stimulated RAW 264.7 and HT-29 cells. To determine the signal pathway involved in the inhibition of NO production, a Western blot analysis was performed. Results showed that OP OFLE decreased phosphorylation of extracellular regulated kinase (pERK) 1/2 and the expression of nuclear factor-kappa B (NF-κB). Our results suggest that extracts of var. leaves and its major components have biological activities such as antioxidative and anti-inflammatory properties.
本研究的目的是确定[植物品种]叶片的最佳提取条件。比较了各种提取物对一氧化氮（NO）产生的抑制作用。使用在对NO产生具有最大影响的最佳提取条件下获得的[植物品种]叶片的各种提取物，对总酚和黄酮含量进行了抗氧化评估。提取[植物品种]叶片的最佳方法得到了一种名为OP OFLE的提取物。OP OFLE以浓度依赖性方式表现出1,1-二苯基-2-三硝基苯肼（DPPH）和2,2'-联氮-双-3-乙基苯并噻唑啉-6-磺酸（ABTS）自由基清除活性。通过高效液相色谱/二极管阵列检测（HPLC/DAD）分析从OP OFLE中分离出主要化合物连翘苷（PH）。在脂多糖（LPS）刺激的RAW 264.7和HT-29细胞中，OP OFLE和PH降低了诱导型一氧化氮合酶（iNOS）和环氧化酶（COX）-2蛋白表达，并下调了促炎细胞因子，如白细胞介素（IL）-1β、IL-6、IL-8和肿瘤坏死因子（TNF）-α。为了确定参与抑制NO产生的信号通路，进行了蛋白质免疫印迹分析。结果表明，OP OFLE降低了细胞外调节蛋白激酶（pERK）1/2的磷酸化以及核因子-κB（NF-κB）的表达。我们的结果表明，[植物品种]叶片提取物及其主要成分具有抗氧化和抗炎等生物活性。
