Balto Hanan A
Department of Restorative Dental Science, King Saud University, College of Dentistry, Riyadh, Kingdom of Saudi Arabia.
J Endod. 2004 Jan;30(1):25-9. doi: 10.1097/00004770-200401000-00005.
The attachment and morphology of human periodontal ligament fibroblasts to mineral trioxide aggregate (MTA) was evaluated using a scanning electron microscope. The material was placed at an apical cavity of 30 single-rooted slices of extracted human teeth. The specimens were divided into two groups of 15 root slices each (freshly mixed and set state). For each experimental group, five root slices were used per observation period (4, 8, and 24 h). A set of two glass slides was used per observation period for the control group. The experiments were performed in tissue-culture cluster 96-well plates in which 1 ml of human periodontal ligament fibroblast cell suspension was placed over the MTA filling and the control glass slides. For the positive-control group, 0.5 ml of methyl methacrylate 2% (vol/vol) was added to the cell suspensions before being dispensed into the wells. Results showed the normal cell morphology in the negative controls. Few round cells with less smooth surfaces and many rough blebs were seen in the positive control, and most of these cells did not show any attachment to the substratum. Similar observations were seen with the freshly prepared-MTA group. In the set-MTA group, cells were round and flattened, displayed smooth surfaces, and appeared to be tightly attached to MTA. It was concluded that the quality and quantity of cell attachment to the retrofilling material could be used as a criterion to evaluate material's toxicity. This research (FN#1077) is registered with the College of Dentistry research center, King Saud University, Riyadh, Saudi Arabia. The author thanks the administration of the King Faisal Specialist Hospital and Research Center, Riyadh, Saudi Arabia, and in particular Dr. M. N. Al-Ahdal for providing the use of the Molecular Virology and Infectious Disease Laboratory, Mr. Yunus Siddiqui for his support, and Dr. Saad AL-Nazhan for his assistance in preparing the manuscript.
使用扫描电子显微镜评估人牙周膜成纤维细胞与三氧化矿物凝聚体（MTA）的附着及形态。将该材料置于30个拔除的人单根牙切片的根尖腔处。标本分为两组，每组15个牙根切片（新鲜混合状态和凝固状态）。对于每个实验组，每个观察期（4、8和24小时）使用5个牙根切片。对照组每个观察期使用一组两片载玻片。实验在组织培养96孔板中进行，其中1毫升人牙周膜成纤维细胞悬液置于MTA填充物和对照载玻片上。对于阳性对照组，在将细胞悬液分配到孔中之前，向其中加入0.5毫升2%（体积/体积）的甲基丙烯酸甲酯。结果显示阴性对照组细胞形态正常。在阳性对照组中可见少数表面不太光滑的圆形细胞和许多粗糙的泡状突起，并且这些细胞中的大多数未显示与基质有任何附着。新鲜制备的MTA组也有类似观察结果。在凝固的MTA组中，细胞呈圆形且扁平，表面光滑，似乎紧密附着于MTA。得出结论，细胞与根充材料附着的质量和数量可作为评估材料毒性的标准。本研究（FN#1077）已在沙特阿拉伯利雅得国王沙特大学牙科学院研究中心注册。作者感谢沙特阿拉伯利雅得法赫德国王专科医院和研究中心的管理部门，尤其感谢M. N. Al-Ahdal博士提供分子病毒学和传染病实验室以供使用，Yunus Siddiqui先生的支持，以及Saad AL-Nazhan博士在准备手稿方面的协助。
