基于γ干扰素的结核病免疫诊断：全血法与酶联免疫斑点法的比较
Gamma interferon-based immunodiagnosis of tuberculosis: comparison between whole-blood and enzyme-linked immunospot methods.
作者信息
Schölvinck Elisabeth, Wilkinson Katalin A, Whelan Adam O, Martineau Adrian R, Levin Michael, Wilkinson Robert J
机构信息
Department of Pediatrics. Wellcome Centre for Research in Clinical Tropical Medicine, Faculty of Medicine, Imperial College London, London W2 1PG.
出版信息
J Clin Microbiol. 2004 Feb;42(2):829-31. doi: 10.1128/JCM.42.2.829-831.2004.
Abstract
The gamma interferon secretion levels of 52 adults whose T cells had been stimulated with the Mycobacterium tuberculosis antigens CFP-10 and ESAT-6 were measured by enzyme-linked immunospot methods and a whole-blood-based assay. The test results correlated well (r = 0.689, P < 0.0001). Its simple format and use of only small volumes of blood make the whole-blood assay suitable for pediatric practice and field trials.
摘要
采用酶联免疫斑点法和全血检测法，对52名用结核分枝杆菌抗原CFP-10和ESAT-6刺激T细胞的成年人的γ干扰素分泌水平进行了检测。检测结果相关性良好（r = 0.689，P < 0.0001）。全血检测法形式简单，仅需少量血液，适用于儿科临床和现场试验。
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