用于诊断侵袭性曲霉病的基于血清的Taqman实时荧光定量PCR检测方法的开发。
Development of a serum-based Taqman real-time PCR assay for diagnosis of invasive aspergillosis.
作者信息
Challier Svetlana, Boyer Stéphanie, Abachin Eric, Berche Patrick
机构信息
Laboratoire de Microbiologie, Hôpital Necker-Enfants Malades, Paris, France.
出版信息
J Clin Microbiol. 2004 Feb;42(2):844-6. doi: 10.1128/JCM.42.2.844-846.2004.
Abstract
We compared an Aspergillus fumigatus-specific Taqman real-time PCR assay for the diagnosis of invasive aspergillosis (IA) with the enzyme-linked immunosorbent assay Platelia Aspergillus method. Patients with proven or probable IA had positive results with at least one of the two methods. The PCR method seems to be more specific, and a combination of the two should improve the diagnosis of IA.
摘要
我们将一种用于诊断侵袭性曲霉病（IA）的烟曲霉特异性Taqman实时聚合酶链反应（PCR）检测方法与酶联免疫吸附测定（ELISA）Platelia曲霉方法进行了比较。确诊或疑似IA的患者使用这两种方法中的至少一种检测结果呈阳性。PCR方法似乎更具特异性，两种方法联合使用应能改善IA的诊断。
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