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用于诊断侵袭性曲霉病的基于血清的Taqman实时荧光定量PCR检测方法的开发。

Development of a serum-based Taqman real-time PCR assay for diagnosis of invasive aspergillosis.

作者信息

Challier Svetlana, Boyer Stéphanie, Abachin Eric, Berche Patrick

机构信息

Laboratoire de Microbiologie, Hôpital Necker-Enfants Malades, Paris, France.

出版信息

J Clin Microbiol. 2004 Feb;42(2):844-6. doi: 10.1128/JCM.42.2.844-846.2004.

DOI:10.1128/JCM.42.2.844-846.2004
PMID:14766869
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC344496/
Abstract

We compared an Aspergillus fumigatus-specific Taqman real-time PCR assay for the diagnosis of invasive aspergillosis (IA) with the enzyme-linked immunosorbent assay Platelia Aspergillus method. Patients with proven or probable IA had positive results with at least one of the two methods. The PCR method seems to be more specific, and a combination of the two should improve the diagnosis of IA.

摘要

我们将一种用于诊断侵袭性曲霉病（IA）的烟曲霉特异性Taqman实时聚合酶链反应（PCR）检测方法与酶联免疫吸附测定（ELISA）Platelia曲霉方法进行了比较。确诊或疑似IA的患者使用这两种方法中的至少一种检测结果呈阳性。PCR方法似乎更具特异性，两种方法联合使用应能改善IA的诊断。

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本文引用的文献

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Development of a LightCycler PCR assay for detection and quantification of Aspergillus fumigatus DNA in clinical samples from neutropenic patients.开发一种用于检测和定量中性粒细胞减少症患者临床样本中烟曲霉DNA的LightCycler聚合酶链反应检测方法。
J Clin Microbiol. 2003 May;41(5):1811-8. doi: 10.1128/JCM.41.5.1811-1818.2003.
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Defining opportunistic invasive fungal infections in immunocompromised patients with cancer and hematopoietic stem cell transplants: an international consensus.定义癌症和造血干细胞移植免疫受损患者中的机会性侵袭性真菌感染：一项国际共识。
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Br J Haematol. 2001 Apr;113(1):180-4. doi: 10.1046/j.1365-2141.2001.02744.x.
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Comparison between plasma and whole blood specimens for detection of Aspergillus DNA by PCR.通过聚合酶链反应（PCR）检测曲霉DNA时血浆与全血标本的比较
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Early detection of aspergillus infection after allogeneic stem cell transplantation by polymerase chain reaction screening.通过聚合酶链反应筛查对异基因造血干细胞移植后曲霉感染进行早期检测。
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Quantification of fungal DNA by using fluorescence resonance energy transfer and the light cycler system.利用荧光共振能量转移和LightCycler系统对真菌DNA进行定量分析。
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