Shen Yuh-Chiang, Chou Cheng-Jen, Wang Yea-Hwey, Chen Chieh-Fu, Chou Yueh-Ching, Lu Mei-Kuang
National Research Institute of Chinese Medicine, Room 739, 155-1, Section 2, Li-Nong Street, Pei-tou District (112), Taipei, Taiwan, ROC.
FEMS Microbiol Lett. 2004 Feb 9;231(1):137-43. doi: 10.1016/S0378-1097(03)00953-4.
We have previously reported that polysaccharides extracted from fruiting bodies or cultured mycelia of Antrodia camphorata exhibit an anti-hepatitis B virus effect. In this study, we intended to elucidate the anti-inflammatory potency of six mycelial extracts, namely PDB-ext, CK-ext, CM-ext, CO-ext, CC-ext, and CKO-ext, isolated from mycelia of A. camphorata cultured with six different media including potato dextrose broth (PDB) and five water-soluble fractions from the wood of different Cinnamomum species, i.e. C. kanehirae (CK), C. micranthum (CM), C. osmophloeum (CO), C. camphora (CC), and C. kotoense (CKO), against reactive oxygen species (ROS) production induced by N-formyl-methionyl-leucyl-phenylalanine (fMLP) or phorbol 12-myristate 13-acetate (PMA) in peripheral human neutrophils (PMN) or mononuclear cells (MNC). ROS produced by PMN or MNC act as inflammatory mediators and also signal immune responses. Pretreatment with these mycelial extracts (1-50 microg ml(-1)) concentration-dependently diminished fMLP- or PMA-induced ROS production in PMN or MNC, as measured by lucigenin-amplified chemiluminescence, with 50% inhibition concentrations (IC(50)) ranging from 2 to 20 microg ml(-1). Among these extracts evaluated, CM-ext, CO-ext, or CKO-ext exhibited higher potency than the others. Using high performance liquid chromatography, we identified two lanostane-type compounds, i.e. dehydrosulfurenic acid and 15alpha-acetyl-dehydrosulfurenic acid, which could be involved in the anti-inflammatory actions of these extracts. The anti-inflammatory actions of these extracts were not due to cytotoxic effects. In summary, these data suggest that extracts from cultured mycelia of A. camphorata display anti-inflammatory effects by inhibiting ROS production in human leukocytes at a pharmacologically applicable concentration. The biological activities of these extracts were further promoted when the culture medium was replaced with water-soluble fractions isolated from the wood of CM, CO or CKO.
我们之前曾报道，从樟芝子实体或培养菌丝体中提取的多糖具有抗乙肝病毒的作用。在本研究中，我们旨在阐明从樟芝菌丝体中分离得到的六种菌丝体提取物的抗炎效力，这六种提取物分别是用六种不同培养基培养樟芝菌丝体得到的，包括马铃薯葡萄糖肉汤（PDB）以及来自不同樟属植物木材的五种水溶性组分，即台湾樟（CK）、细叶香桂（CM）、沉水樟（CO）、樟树（CC）和台东樟（CKO），研究它们对人外周血中性粒细胞（PMN）或单核细胞（MNC）中由N - 甲酰 - 甲硫氨酰 - 亮氨酰 - 苯丙氨酸（fMLP）或佛波酯12 - 肉豆蔻酸酯13 - 乙酸酯（PMA）诱导产生的活性氧（ROS）的影响。PMN或MNC产生的ROS作为炎症介质，也参与免疫反应信号传导。用这些菌丝体提取物（1 - 50 μg ml⁻¹）进行预处理，通过光泽精增强化学发光法测定，可浓度依赖性地减少fMLP或PMA诱导的PMN或MNC中ROS的产生，半数抑制浓度（IC₅₀）范围为2至20 μg ml⁻¹。在评估的这些提取物中，CM - ext、CO - ext或CKO - ext表现出比其他提取物更高的效力。通过高效液相色谱法，我们鉴定出两种羊毛甾烷型化合物，即脱氢硫辛酸和15α - 乙酰 - 脱氢硫辛酸，它们可能参与了这些提取物的抗炎作用。这些提取物的抗炎作用并非由于细胞毒性作用。总之，这些数据表明，樟芝培养菌丝体的提取物在药理学适用浓度下通过抑制人白细胞中ROS的产生而发挥抗炎作用。当培养基被CM、CO或CKO木材中分离出的水溶性组分替代时，这些提取物的生物活性进一步增强。
