嗅球去甲肾上腺素在早期嗅觉学习中的作用。
The role of olfactory bulb norepinephrine in early olfactory learning.
作者信息
Sullivan R M, Zyzak D R, Skierkowski P, Wilson D A
机构信息
Department of Psychology, University of Oklahoma, Norman 73019.
出版信息
Brain Res Dev Brain Res. 1992 Dec 18;70(2):279-82. doi: 10.1016/0165-3806(92)90207-d.
Wistar rat pups were implanted with bilateral olfactory bulb cannulas on postnatal day 5 (PN5). On PN6, pups were trained in an olfactory classical conditioning task with peppermint odor as the CS and tactile stimulation/stroking as the UCS. Pups were randomly assigned to either PAIRED, BACKWARD or ODOR-only conditions. Half the pups in each group received intrabulbar infusions of 100 microM propranolol and half received intrabulbar infusions of saline during the training session. Propranolol infusions blocked acquisition of the learned odor preference expressed by PAIRED saline-infused pups. Diffusion of the infusate was checked in additional pups by infusing [3H]NE and performing LSC analysis. Infusate concentration did not significantly differ between the anterior and posterior halves of the bulb, but were sharply lower in the olfactory peduncle and more posterior areas. The results suggest that olfactory bulb NE is critical for early olfactory learning.
在出生后第5天（PN5），将双侧嗅球插管植入Wistar大鼠幼崽体内。在PN6时，用薄荷气味作为条件刺激（CS），触觉刺激/抚摸作为非条件刺激（UCS），对幼崽进行嗅觉经典条件反射任务训练。幼崽被随机分配到配对组、反向组或仅气味组。每组中有一半的幼崽在训练期间接受球内注射100微摩尔普萘洛尔，另一半接受球内注射生理盐水。普萘洛尔注射阻断了接受生理盐水注射的配对幼崽所表现出的习得气味偏好的获得。通过注射[3H]去甲肾上腺素（NE）并进行液体闪烁计数（LSC）分析，在另外的幼崽中检查注入物的扩散情况。注入物浓度在嗅球的前半部分和后半部分之间没有显著差异，但在嗅柄和更靠后的区域明显较低。结果表明，嗅球去甲肾上腺素对早期嗅觉学习至关重要。
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