Grosz M D, Womack J E, Skow L C
Department of Veterinary Anatomy and Public Health, Texas A&M University, College Station 77843.
Genomics. 1992 Dec;14(4):863-8. doi: 10.1016/s0888-7543(05)80106-5.
A phage library of bovine genomic DNA was screened for hybridization with a human HSP70 cDNA probe, and 21 positive plaques were identified and isolated. Restriction mapping and blot hybridization analysis of DNA from the recombinant plaques demonstrated that the cloned DNAs were derived from three different regions of the bovine genome. One region contains two tandemly arrayed HSP70 sequences, designated HSP70-1 and HSP70-2, separated by approximately 8 kb of DNA. Single HSP70 sequences, designated HSP70-3 and HSP70-4, were found in two other genomic regions. Locus-specific probes of unique flanking sequences from representative HSP70 clones were hybridized to restriction endonuclease-digested DNA from bovine-hamster and bovine-mouse somatic cell hybrid panels to determine the chromosomal location of the HSP70 sequences. The probe for the tandemly arrayed HSP70-1 and HSP70-2 sequences mapped to bovine chromosome 23, syntenic with glyoxalase 1, 21 steroid hydroxylase, and major histocompatibility class I loci. HSP70-3 sequences mapped to bovine chromosome 10, syntenic with nucleoside phosphorylase and murine osteosarcoma viral oncogene (v-fos), and HSP70-4 mapped to bovine syntenic group U6, syntenic with amylase 1 and phosphoglucomutase 1. On the basis of these data, we propose that bovine HSP70-1,2 are homologous to human HSPA1 and HSPA1L on chromosome 6p21.3, bovine HSP70-3 is the homolog of an unnamed human HSP70 gene on chromosome 14q22-q24, and bovine HSP70-4 is homologous to one of the human HSPA-6,-7 genes on chromosome 1.
用人HSP70 cDNA探针筛选牛基因组DNA噬菌体文库，鉴定并分离出21个阳性噬菌斑。对重组噬菌斑的DNA进行限制性图谱分析和印迹杂交分析，结果表明克隆的DNA来自牛基因组的三个不同区域。一个区域包含两个串联排列的HSP70序列，命名为HSP70-1和HSP70-2，中间间隔约8 kb的DNA。在另外两个基因组区域发现了单个HSP70序列，命名为HSP70-3和HSP70-4。用来自代表性HSP70克隆的独特侧翼序列的位点特异性探针与牛-仓鼠和牛-小鼠体细胞杂交板经限制性内切酶消化的DNA杂交，以确定HSP70序列的染色体定位。串联排列的HSP70-1和HSP70-2序列的探针定位于牛的23号染色体，与乙二醛酶1、21-类固醇羟化酶和主要组织相容性复合体I类基因座同线。HSP70-3序列定位于牛的10号染色体，与核苷磷酸化酶和鼠骨肉瘤病毒癌基因（v-fos）同线，HSP70-4定位于牛的同线群U6，与淀粉酶1和磷酸葡萄糖变位酶1同线。根据这些数据我们推测，牛HSP70-1,2与人类6号染色体p21.3上的HSPA1和HSPA1L同源，牛HSP70-3是人类14号染色体q22-q24上一个未命名的HSP70基因的同源物，牛HSP70-4与人类1号染色体上的HSPA-6,-7基因之一同源。
