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通过简单的双板薄层色谱法从脱氢多萜醇中分离多萜醇。

Separation of dolichol from dehydrodolichol by a simple two-plate thin-layer chromatography.

作者信息

Sagami H, Kurisaki A, Ogura K, Chojnacki T

机构信息

Institute for Chemical Reaction Science, Tohoku University, Sendai, Japan.

出版信息

J Lipid Res. 1992 Dec;33(12):1857-61.

PMID:1479294
Abstract

A novel thin-layer chromatographic procedure was devised to separate dolichol and dehydrodolichol from each other with the concomitant separation of each family with respect to the carbon chain length. This method involves development of the polyprenols successively on two different plates, a silica gel plate and a reversed-phase plate.

摘要

设计了一种新型薄层色谱法,用于分离多萜醇和脱氢多萜醇,同时根据碳链长度对每个家族进行分离。该方法包括在两块不同的板上依次展开聚萜烯醇,一块硅胶板和一块反相板。

相似文献

1
Separation of dolichol from dehydrodolichol by a simple two-plate thin-layer chromatography.通过简单的双板薄层色谱法从脱氢多萜醇中分离多萜醇。
J Lipid Res. 1992 Dec;33(12):1857-61.
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Enzymatic formation of dehydrodolichal and dolichal, new products related to yeast dolichol biosynthesis.脱氢多萜醇和多萜醇的酶促形成,与酵母多萜醇生物合成相关的新产物。
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Formation of dolichol from dehydrodolichol is catalyzed by NADPH-dependent reductase localized in microsomes of rat liver.由脱氢多萜醇形成多萜醇是由位于大鼠肝脏微粒体中的NADPH依赖性还原酶催化的。
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Separation, quantitation and distribution of dolichol and dolichyl phosphate in rat and human tissues.大鼠和人体组织中多萜醇及磷酸多萜醇的分离、定量与分布
Biochim Biophys Acta. 1983 May 16;751(3):355-68. doi: 10.1016/0005-2760(83)90294-1.
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Enrichment of the intracellular dolichol pool in isolated liver cells.
J Lipid Res. 1982 Sep;23(7):972-83.
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Separation and quantitative determination of dolichol and dolichyl phosphate in rat and trout liver.大鼠和鳟鱼肝中多萜醇及磷酸多萜醇的分离与定量测定。
Comp Biochem Physiol B. 1986;84(4):565-9. doi: 10.1016/0305-0491(86)90124-0.
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Single polyprenol and dolichol isolation by semipreparative high-performance liquid chromatography technique.通过半制备高效液相色谱技术分离单聚戊烯醇和多萜醇
J Lipid Res. 2000 Jul;41(7):1177-80.
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The half-lives of dolichol and dolichyl phosphate in rat liver.大鼠肝脏中多萜醇和磷酸多萜醇的半衰期。
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The uptake of dietary polyprenols and their modification to active dolichols by the rat liver.大鼠肝脏对膳食多萜醇的摄取及其向活性多萜醇的转化。
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