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用于评估体外受精失败的精子电子显微镜检查

Sperm electron microscopy for the evaluation of in vitro fertilization failures.

作者信息

Nulsen J C, Metzger D A, Steinhoff M M

机构信息

Department of Obstetrics and Gynecology, University of Connecticut Health Center, Farmington 06030.

出版信息

J Assist Reprod Genet. 1992 Oct;9(5):491-7. doi: 10.1007/BF01204058.

DOI:10.1007/BF01204058
PMID:1482847
Abstract

When performing IVF, the clinician is frequently confronted with the failure of fertilization. When the standard parameters to evaluate the male factor are "within normal limits," the conclusion is often made that the lack of fertilization is most likely due to "poor egg quality." These two cases demonstrate the fallacy of this approach and support a more rigorous evaluation of the male factor. Ultrastructural analysis of sperm is underutilized and, as demonstrated by these two cases, can play an essential role in this evaluation process.

摘要

在进行体外受精(IVF)时,临床医生经常面临受精失败的情况。当评估男性因素的标准参数“在正常范围内”时,往往会得出受精失败很可能是由于“卵子质量差”的结论。这两个案例证明了这种方法的错误,并支持对男性因素进行更严格的评估。精子的超微结构分析未得到充分利用,而这两个案例表明,它在这个评估过程中可以发挥重要作用。

相似文献

1
Sperm electron microscopy for the evaluation of in vitro fertilization failures.用于评估体外受精失败的精子电子显微镜检查
J Assist Reprod Genet. 1992 Oct;9(5):491-7. doi: 10.1007/BF01204058.
2
Nuclear decondensation of sperm head and failure at in-vitro fertilization: an ultrastructural study.
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Sperm chromatin structure assay (SCSA) parameters are related to fertilization, blastocyst development, and ongoing pregnancy in in vitro fertilization and intracytoplasmic sperm injection cycles.精子染色质结构分析(SCSA)参数与体外受精和卵胞浆内单精子注射周期中的受精、囊胚发育及持续妊娠相关。
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Sperm chromatin structure assay parameters are not related to fertilization rates, embryo quality, and pregnancy rates in in vitro fertilization and intracytoplasmic sperm injection, but might be related to spontaneous abortion rates.精子染色质结构分析参数与体外受精和卵胞浆内单精子注射的受精率、胚胎质量及妊娠率无关,但可能与自然流产率有关。
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Ultrastructural sperm investigations in normospermic subjects with repeated failures of oocyte fertilizations.对卵母细胞反复受精失败的正常精子受试者进行超微结构精子研究。
Acta Eur Fertil. 1992 Nov-Dec;23(6):297-300.

本文引用的文献

1
Ability of antibody-bound human sperm to penetrate zona-free hamster ova in vitro.体外抗体结合的人精子穿透无透明带仓鼠卵的能力。
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The zona-free hamster egg penetration assay as a prognostic indicator in a human in vitro fertilization program.无透明带仓鼠卵穿透试验作为人类体外受精程序中的一项预后指标。
Fertil Steril. 1985 Mar;43(3):433-7. doi: 10.1016/s0015-0282(16)48444-x.
6
Inner acrosomal membrane of mammalian spermatozoa: its properties and possible functions in fertilization.哺乳动物精子顶体膜内部:其特性及在受精过程中的可能功能。
Am J Anat. 1985 Nov;174(3):249-68. doi: 10.1002/aja.1001740307.
7
Human sperm penetration assay as an indicator of sperm function in human in vitro fertilization.
Fertil Steril. 1987 Aug;48(2):282-6. doi: 10.1016/s0015-0282(16)59357-1.
8
The ultrastructural pathology of the spermatozoon as a cause of infertility: the role of electron microscopy in the evaluation of semen quality.作为不育原因的精子超微结构病理学:电子显微镜在精液质量评估中的作用。
Fertil Steril. 1987 Nov;48(5):711-34. doi: 10.1016/s0015-0282(16)59520-x.
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The gamete membrane fusion test to assay the fertilizing ability of human spermatozoa.用于测定人类精子受精能力的配子膜融合试验。
Hum Reprod. 1988 Jul;3(5):637-44. doi: 10.1093/oxfordjournals.humrep.a136759.
10
A comparative analysis of the cycle fecundity rates associated with combined human menopausal gonadotropin (hMG) and intrauterine insemination (IUI) versus either hMG or IUI alone.对与联合使用人绝经期促性腺激素(hMG)和宫腔内人工授精(IUI)相比单独使用hMG或IUI相关的周期受孕率进行的比较分析。
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