Gast R K, Stone H D, Holt P S, Beard C W
United States Department of Agriculture, Southeast Poultry Research Laboratory, Athens, Georgia 30605.
Avian Dis. 1992 Oct-Dec;36(4):992-9.
To assess the potential protective efficacy of a Salmonella enteritidis bacterin, an acetone-killed oil-emulsion vaccine was prepared from a phage type 13a S. enteritidis strain and administered subcutaneously to hens in two experiments. Hens were housed individually, and every other hen was vaccinated (at 23 weeks of age in one experiment and at 45 weeks in the other). A second (booster) bacterin injection was administered 6 weeks later in both experiments. Three weeks after the second vaccination, all hens were challenged with an oral dose of approximately 10(9) cells of a heterologous (phage type 14b) S. enteritidis strain. In both trials, S. enteritidis was isolated from fewer internal organs (spleens, ovaries, and oviducts) and pools of egg contents from vaccinated hens than from unvaccinated control hens. Vaccination did not, however, affect the percentage of hens that shed S. enteritidis in feces in either experiment.
为评估肠炎沙门氏菌菌苗的潜在保护效力，用13a型肠炎沙门氏菌菌株制备了一种丙酮灭活油乳剂疫苗，并在两项实验中对母鸡进行皮下接种。母鸡单独饲养，每隔一只母鸡接种疫苗（一项实验中在23周龄时接种，另一项实验中在45周龄时接种）。两项实验均在6周后进行第二次（加强）菌苗注射。第二次接种疫苗三周后，所有母鸡口服约10⁹个异源（14b型噬菌体）肠炎沙门氏菌菌株细胞进行攻毒。在两项试验中，与未接种疫苗的对照母鸡相比，接种疫苗的母鸡体内脏器（脾脏、卵巢和输卵管）以及蛋内容物中分离出的肠炎沙门氏菌数量更少。然而，在任何一项实验中，接种疫苗均未影响粪便中排出肠炎沙门氏菌的母鸡比例。
