Ookusa Y, Takata K, Nagashima M, Hirano H
Histochemistry. 1983;79(1):1-7. doi: 10.1007/BF00494336.
Lectin binding patterns in normal human skin were studied using five different biotinyl lectins and avidin-horseradish peroxidase. The staining pattern was specific for each lectin. In the epidermis, peanut agglutinin (PNA) and soybean agglutinin (SBA) preferentially stained the cell membranes of keratinocytes in the spinous and granular cell layers, indicating changes in the saccharide residues during keratinocyte differentiation. In the secretory segment of an eccrine sweat gland, the superficial cells gave a strong granular staining with Ricinus communis agglutinin (RCA). Dolichos biflorus agglutinin (DBA) and SBA, on the other hand, strongly stained the basal cells. With these lectins, two types of cells in the secretory segment were clearly distinguished. These results show that (1) PNA and SBA binding sites increase during the course of keratinocyte differentiation, and (2) RCA, DBA, and SBA are good markers to distinguish two types of cells in the secretory segment of an eccrine sweat gland.
利用五种不同的生物素化凝集素和抗生物素蛋白-辣根过氧化物酶研究了正常人皮肤中的凝集素结合模式。每种凝集素的染色模式都是特异的。在表皮中，花生凝集素（PNA）和大豆凝集素（SBA）优先染色棘层和颗粒层角质形成细胞的细胞膜，表明角质形成细胞分化过程中糖残基的变化。在小汗腺的分泌段，表层细胞用蓖麻凝集素（RCA）呈现强烈的颗粒状染色。另一方面，双花扁豆凝集素（DBA）和SBA强烈染色基底细胞。使用这些凝集素，可以清楚地区分分泌段的两种细胞类型。这些结果表明：（1）角质形成细胞分化过程中PNA和SBA结合位点增加；（2）RCA、DBA和SBA是区分小汗腺分泌段两种细胞类型的良好标记物。
