Datta S, Calvo J M, Quattrochi J, Hobson J A
Laboratory of Neurophysiology, Harvard Medical School, Boston, Massachusetts 02115.
Arch Ital Biol. 1992 Oct;130(4):263-84.
The cholinergic agonist carbachol was injected into the pontine Pb area where PGO bursting cells have been recorded. When microinjections were localized to the ventrolateral aspect of the caudal Pb nucleus near aggregates of ChAT immunolabeled cholinergic neurons, carbachol produced an immediate onset of state-independent PGO waves in the ipsilateral LGB. These state-independent PGO waves persisted for 3-4 days. After the first 24 hrs PGO wave activity increasingly became associated with REM sleep and with REM transitional SP sleep as both of these PGO-related states increased in amount to 3-4 times baseline levels. The increase in amount of PGO-related states peaked on days 2-4 following one carbachol injection and persisted for 10-12 days. These results suggest a two stage process: stage one, PGO enhancement, is the direct consequence of the membrane activation of cholinoceptive PGO burst neurons by carbachol; stage two, REM enhancement, is the consequence of metabolic activation of endogenous cholinergic neurons. This experimental preparation is a useful model for the study of the electrophysiology and functional significance of PGO wave and REM sleep generation.
将胆碱能激动剂卡巴胆碱注射到已记录到脑桥PGO爆发细胞的脑桥Pb区。当微量注射定位在靠近ChAT免疫标记胆碱能神经元聚集处的尾侧Pb核腹外侧时，卡巴胆碱在同侧外侧膝状体立即引发与状态无关的PGO波。这些与状态无关的PGO波持续3 - 4天。在最初的24小时后，PGO波活动越来越多地与快速眼动睡眠以及快速眼动过渡性慢波睡眠相关，因为这两种与PGO相关的状态数量增加到基线水平的3 - 4倍。一次卡巴胆碱注射后，与PGO相关状态的数量增加在第2 - 4天达到峰值，并持续10 - 12天。这些结果表明存在两个阶段的过程：第一阶段，PGO增强，是卡巴胆碱对胆碱能感受性PGO爆发神经元进行膜激活的直接结果；第二阶段，快速眼动增强，是内源性胆碱能神经元代谢激活的结果。这种实验制剂是研究PGO波和快速眼动睡眠产生的电生理学及功能意义的有用模型。
