Datta S, Calvo J M, Quattrochi J J, Hobson J A
Laboratory of Neurophysiology, Harvard Medical School, Boston, MA 02115.
Neuroreport. 1991 Oct;2(10):619-22. doi: 10.1097/00001756-199110000-00017.
A six day long increase in rapid eye movement (REM) sleep followed the unilateral microinjection of a single dose of the cholinergic agonist drug carbachol into the brain stem of cats. Effective drug injection sites were localized to the pontine peribrachial region containing cholinergic choline acetyltransferase (ChAT) labeled neurons. At the peak of the effect, which occurred 24-28 h post-injection, the relative amount of time devoted to REM sleep tripled, resulting in an absolute time increase from 3.12 to 11.28 h REM sleep per day. This pronounced and prolonged REM sleep increase was associated with marked enhancement of ponto-geniculo-occipital (PGO) waves and with PGO burst cell activity unilateral to the site of injection.
向猫的脑干单侧微量注射单剂量胆碱能激动剂药物卡巴胆碱后，快速眼动（REM）睡眠持续增加了六天。有效的药物注射部位定位于含有胆碱能胆碱乙酰转移酶（ChAT）标记神经元的脑桥臂周区域。在注射后24 - 28小时出现效应峰值时，用于REM睡眠的相对时间增加了两倍，导致每天REM睡眠的绝对时间从3.12小时增加到11.28小时。这种显著且持久的REM睡眠增加与脑桥 - 膝状体 - 枕叶（PGO）波的显著增强以及注射部位单侧的PGO爆发细胞活动有关。
