固定化细胞生长因子上的细胞生长；4：成纤维细胞与固定在聚甲基丙烯酸甲酯膜上的胰岛素的相互作用。

Cell growth on immobilized cell-growth factor; 4: Interaction of fibroblast cells with insulin immobilized on poly(methyl methacrylate) membrane.

作者信息

Liu S Q, Ito Y, Imanishi Y

机构信息

Department of Polymer Chemistry, Faculty of Engineering, Kyoto University, Japan.

出版信息

J Biochem Biophys Methods. 1992 Oct;25(2-3):139-48. doi: 10.1016/0165-022x(92)90006-v.

DOI:10.1016/0165-022x(92)90006-v

PMID:1491099

Abstract

Insulin was immobilized on the surface-hydrolyzed poly(methyl methacrylate) membrane and the growth acceleration of mouse fibroblast cells, STO, by the immobilized insulin was investigated. It was found that insulin remains immobilized on the surface of nonbiodegradable membrane and interacts specifically with receptors existing on the biological membrane of fibroblast cells. The growth acceleration by immobilized insulin was enhanced by introduction of a spacer arm between insulin and the immobilization matrix. The amount of receptor proteins present on the biological membrane of fibroblast cells after culturing with insulin, immobilized on nonbiodegradable polymer membrane, was much higher than that after culturing with free insulin, implying the suppression of down-regulation in the case of immobilized insulin.

摘要

将胰岛素固定在表面水解的聚甲基丙烯酸甲酯膜上，并研究固定化胰岛素对小鼠成纤维细胞STO生长的促进作用。结果发现，胰岛素保留在不可生物降解膜的表面，并与成纤维细胞生物膜上存在的受体特异性相互作用。通过在胰岛素和固定化基质之间引入间隔臂，增强了固定化胰岛素对生长的促进作用。在用固定在不可生物降解聚合物膜上的胰岛素培养后，成纤维细胞生物膜上存在的受体蛋白量远高于用游离胰岛素培养后的量，这意味着在固定化胰岛素的情况下抑制了下调。