Liu S Q, Ito Y, Imanishi Y
Department of Polymer Chemistry, Faculty of Engineering, Kyoto University, Japan.
Int J Biol Macromol. 1993 Aug;15(4):221-6. doi: 10.1016/0141-8130(93)90041-j.
Transferrin was immobilized on poly(methyl methacrylate) membrane and the interaction of the immobilized transferrin with mouse fibroblast cells STO was investigated. The immobilized transferrin was stable without decomposition over 48 h and showed higher activities of cell growth acceleration than free transferrin and transported ferric ion as effectively as free transferrin. The specific interaction of the immobilized transferrin with fibroblast cells was confirmed by experiment using the antibody. It was considered that cell growth acceleration and ferric ion transportation occur by different mechanisms.
转铁蛋白固定在聚甲基丙烯酸甲酯膜上，并研究了固定化转铁蛋白与小鼠成纤维细胞STO的相互作用。固定化转铁蛋白在48小时内稳定无分解，与游离转铁蛋白相比，具有更高的细胞生长促进活性，并且能像游离转铁蛋白一样有效地转运铁离子。通过使用抗体的实验证实了固定化转铁蛋白与成纤维细胞的特异性相互作用。据认为，细胞生长促进和铁离子转运是通过不同机制发生的。
