Griebel P J, Kennedy L, Graham T, Davis W C, Reynolds J D
Department of Medical Physiology, Health Sciences Centre, University of Calgary, Alberta, Canada.
Immunology. 1992 Dec;77(4):564-70.
Changes in B-cell phenotype during development of ileal and jejunal Peyer's patches (PP) of sheep were investigated using flow cytometry and immunoperoxidase-stained cryosections. On Day 104 of gestation (term at 150 days) B-cell clusters were identified in the lamina propria of the ileum. These clusters were composed of cells that expressed surface IgM (sIgM), lambda or kappa light chain, and BAQ44A, a B-cell differentiation molecule. No cells in the clusters stained for terminal deoxynucleotide transferase. On Day 132 gestation, a change was evident in the phenotype of ileal PP B cells. Most B cells expressed a reduced level of sIgM and 20% were BAQ44A-. The B cells in the dome region were BAQ44A+ but few BAQ44A+ cells were present in the follicles. At 6-8 weeks of age BAQ44A+ cells were restricted to the dome region of the ileal PP; flow cytometric analysis confirmed that 25% of B cells isolated from the dome/follicle complex were BAQ44A+. Thus, the primordial PP was populated with B cells that were phenotypically similar to circulating B cells (sIgMhigh, BAQ44A+). After 132 days gestation, the predominant B-cell phenotype in the ileal PP changed to sIgMlow and BAQ44A-. This phenotypic change could be the result of either early immigrant B-cell differentiation or subsequent colonization by sIgMlow BAQ44A- B cells. The phenotypic changes of ileal PP follicular B cells were not complete until after birth and different phenotypic changes were observed in follicles of the jejunal PP of young lambs.
利用流式细胞术和免疫过氧化物酶染色冷冻切片，研究了绵羊回肠和空肠派尔集合淋巴结（PP）发育过程中B细胞表型的变化。在妊娠第104天（妊娠期为150天），在回肠固有层中发现了B细胞簇。这些细胞簇由表达表面IgM（sIgM）、λ或κ轻链以及B细胞分化分子BAQ44A的细胞组成。细胞簇中没有细胞对末端脱氧核苷酸转移酶染色呈阳性。在妊娠第132天，回肠PP B细胞的表型出现明显变化。大多数B细胞表达的sIgM水平降低，20%的细胞BAQ44A呈阴性。圆顶区域的B细胞BAQ44A呈阳性，但卵泡中几乎没有BAQ44A阳性细胞。在6 - 8周龄时，BAQ44A阳性细胞局限于回肠PP的圆顶区域；流式细胞术分析证实，从圆顶/卵泡复合体中分离出的B细胞中有25%是BAQ44A阳性。因此，原始PP中充满了表型与循环B细胞相似的B细胞（sIgM高，BAQ44A阳性）。妊娠132天后，回肠PP中主要的B细胞表型变为sIgM低和BAQ44A阴性。这种表型变化可能是早期迁入的B细胞分化的结果，也可能是sIgM低BAQ44A阴性B细胞随后定植的结果。回肠PP卵泡B细胞的表型变化直到出生后才完成，并且在幼龄羔羊空肠PP的卵泡中观察到了不同的表型变化。
