Abrahamsen M S, Lancto C A, Walcheck B, Layton W, Jutila M A
Department of Veterinary PathoBiology, University of Minnesota, St. Paul 55108, USA.
Infect Immun. 1997 Jun;65(6):2428-33. doi: 10.1128/iai.65.6.2428-2433.1997.
The nature of the host's T-lymphocyte population within the intestinal villi following Cryptosporidium parvum infection was characterized with a bovine model of cryptosporidiosis. In naive animals, infection with C. parvum resulted in substantial increases in the numbers of alpha/beta T cells, both CD4+ (150%) and CD8+ (60%), and of gamma/delta T cells (70%) present within the intestinal villi of the infected ileum. In immune animals, the host T-lymphocyte response to a challenge infection with C. parvum was restricted to alpha/beta T cells. The number of CD4+ T cells within the Peyer's patch of the ileum increased dramatically; however, there was little change in the number or localization of CD4+ T cells within the intestinal villi. In contrast, the number of CD8+ T cells within the intestinal villi increased following a challenge infection. In addition, the CD8+ T cells were found to be intimately associated with the epithelial cells of the intestinal villi. The precise correlation between the accumulation of CD8+ T cells and the normal site of parasite development suggests an important role for CD8+ T cells in the immune animal.
利用隐孢子虫病的牛模型，对微小隐孢子虫感染后肠绒毛内宿主T淋巴细胞群体的性质进行了表征。在未感染的动物中，微小隐孢子虫感染导致感染回肠肠绒毛内α/β T细胞数量大幅增加，CD4⁺ T细胞增加150%，CD8⁺ T细胞增加60%，γ/δ T细胞增加70%。在免疫动物中，宿主对微小隐孢子虫攻击感染的T淋巴细胞反应仅限于α/β T细胞。回肠派尔集合淋巴结内CD4⁺ T细胞数量急剧增加；然而，肠绒毛内CD4⁺ T细胞的数量或定位几乎没有变化。相比之下，攻击感染后肠绒毛内CD8⁺ T细胞数量增加。此外，发现CD8⁺ T细胞与肠绒毛的上皮细胞密切相关。CD8⁺ T细胞的积累与寄生虫发育的正常部位之间的确切相关性表明CD8⁺ T细胞在免疫动物中发挥重要作用。
