SCHAEFER W B
J Exp Med. 1952 Sep;96(3):207-19. doi: 10.1084/jem.96.3.207.
The nutritional requirements of dysgonic and eugonic bovine tubercle bacilli were investigated in liquid Tween-albumin medium. The following conclusions were reached:- Dysgonic bovine tubercle bacilli require for multiplication long chain fatty acids which can be supplied as oleic, palmitic, or stearic acid whereas eugonic bovine strains can grow in the absence of fatty acid and can utilize glucose as a sufficient source of carbon. In the presence of fatty acids, the growth of dysgonic strains is increased by the addition of small amounts of glucose or glycerol, whereas large amounts of glucose or glycerol decrease the total growth and inhibit or retard the growth of small inocula. In glucose-fatty acid media, the growth of dysgonic strains is further stimulated by the addition of sodium glutamate, succinate, malate, or acetate. Sodium pyruvate enables dysgonic strains to grow rapidly in liquid media containing glucose or glycerol even in the absence of fatty acid. The significance of these findings for the mechanism of the variation from "dysgonic" to "eugonic" is discussed. The use of oleic acid-albumin agar without glucose permits the rapid isolation of bovine tubercle bacilli from natural sources.
在吐温 - 白蛋白液体培养基中研究了生长不良型和生长良好型牛结核杆菌的营养需求。得出以下结论：生长不良型牛结核杆菌繁殖需要长链脂肪酸，可提供油酸、棕榈酸或硬脂酸，而生长良好型牛结核杆菌菌株在无脂肪酸的情况下也能生长，并且可以利用葡萄糖作为充足的碳源。在有脂肪酸存在时，添加少量葡萄糖或甘油可促进生长不良型菌株的生长，而大量葡萄糖或甘油会减少总生长量，并抑制或延缓小接种量的生长。在葡萄糖 - 脂肪酸培养基中，添加谷氨酸钠、琥珀酸盐、苹果酸盐或乙酸盐可进一步刺激生长不良型菌株的生长。即使在没有脂肪酸的情况下，丙酮酸钠也能使生长不良型菌株在含有葡萄糖或甘油的液体培养基中快速生长。讨论了这些发现对于从“生长不良型”到“生长良好型”变异机制的意义。使用不含葡萄糖的油酸 - 白蛋白琼脂可从天然来源快速分离牛结核杆菌。
