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[正常儿童口腔中念珠菌属的分布]

[The distribution of Candida spp. in the oral cavities of normal children].

作者信息

Qi Qing-guo, Wang Wen-xia, Zhou Xue-dong, Wang Duo, Xiao Xiao-rong

机构信息

Stomatology College of Shandong University, Jinan, Shandong 250012, China.

出版信息

Shanghai Kou Qiang Yi Xue. 2003 Aug;12(4):288-91.

Abstract

OBJECTIVE

To study the distribution of Candida spp. mainly Candida albicans in the oral cavities of health children.

METHODS

Four groups children of different ages, A1: newborn babies, A2: 3.2 years old (average) children,A3, 7.2 years old,A4:12.7 years old and B control group 20.4 years old,mucosal swab sampling with centrification,CHROMagar Candida identified culture medium for culture and identification, and different methods for isolating Candida albicans for A3 group.

RESULTS

The isolation rates of Candida spp were A1 7.5%,A2 70%,A3 56.36%,A4 49.12%,B 27.5%,the proportion of Candida albicans also diversely,and the method of PCR was more sensitive than the one of culture.

CONCLUSION

Candida spp can be isolated from the normal oral cavities in different aged children, most of them were Candida albicans,both the isolation rates of Candida spp and the proportion of Candida albicans were different.

摘要

目的

研究念珠菌属,主要是白色念珠菌在健康儿童口腔中的分布情况。

方法

选取四组不同年龄段的儿童,A1组:新生儿;A2组:平均年龄3.2岁的儿童;A3组:7.2岁的儿童;A4组:12.7岁的儿童;以及B对照组:20.4岁的成年人。采用黏膜拭子采样并离心,使用科玛嘉念珠菌显色培养基进行培养和鉴定,对A3组采用不同方法分离白色念珠菌。

结果

念珠菌属的分离率分别为:A1组7.5%,A2组70%,A3组56.36%,A4组49.12%,B组27.5%,白色念珠菌的比例也各不相同,且聚合酶链反应(PCR)方法比培养法更敏感。

结论

不同年龄段儿童的正常口腔中均可分离出念珠菌属,其中大部分为白色念珠菌,念珠菌属的分离率和白色念珠菌的比例均有所不同。

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