Beck Frances W J, Prasad Ananda S, Butler Charles E, Sakr Wael A, Kucuk Omer, Sarkar Fazlul H
Department of Internal Medicine, Division of Hematology/Oncology and Karmanos Cancer Institute, Wayne State University School of Medicine, Detroit, Michigan 48201, USA.
Prostate. 2004 Mar 1;58(4):374-81. doi: 10.1002/pros.10344.
Altered zinc levels in prostate benign prostatic hyperplasia (BPH) and carcinoma is well documented. It is not known whether loss of zinc, necessary to restrain aggressive growth, results from loss of a single specific or multiple zinc transporters.
Human prostate tissues from patients who underwent radical prostatectomy were screened by RT-PCR analysis for five zinc transporters. Relative cDNA expression was determined in normal, BPH, and tumor specimens and four prostate epithelial cell lines.
Surgical specimens were obtained from patients with assigned Gleason scores ranging from 5 to 9. Relative expression of hZIP-1 and hZnT-1 were prominent in most samples with relatively lesser degree of expression of hZIP-2 and no detectable expression of hZnT-3. Expression of hZnT-4 was decreased in BPH and tumor samples compared to normal tissue.
These data suggest that zinc homeostasis in normal prostate tissues results from an interplay of multiple transporters and decreased hZnT-4 expression is associated with prostate tissue abnormalities independent of total cellular zinc content.
前列腺良性前列腺增生（BPH）和癌组织中锌水平的改变已有充分记录。目前尚不清楚抑制侵袭性生长所需的锌的丢失是由单一特定锌转运蛋白的丢失还是多种锌转运蛋白的丢失导致的。
对接受根治性前列腺切除术患者的人前列腺组织进行逆转录聚合酶链反应（RT-PCR）分析，以检测五种锌转运蛋白。在正常、BPH和肿瘤标本以及四种前列腺上皮细胞系中测定相对cDNA表达。
手术标本取自Gleason评分在5至9之间的患者。hZIP-1和hZnT-1的相对表达在大多数样本中较为突出，hZIP-2的表达程度相对较低，且未检测到hZnT-3的表达。与正常组织相比，BPH和肿瘤样本中hZnT-4的表达降低。
这些数据表明，正常前列腺组织中的锌稳态是由多种转运蛋白相互作用产生的，hZnT-4表达降低与前列腺组织异常有关，且与细胞总锌含量无关。
