McConnachie P R, Zahalsky A C
Immunotransplant Laboratory Memorial Medical Center, Springfield, Illinois.
Arch Environ Health. 1992 Jul-Aug;47(4):295-301. doi: 10.1080/00039896.1992.9938365.
Lymphocyte phenotype frequencies and in vitro functional assays were studied in 27 individuals who had been exposed to technical chlordane in their homes or at their places of work. A control group consisted of 118 individuals who were similar to the exposed group with respect to age and sex distribution, and who had not knowingly experienced exposure to technical chlordane, was chosen for study. A significantly increased frequency of cortical thymocytes in the circulation (CD1) (p less than .001) and a decreased frequency of the suppressor-inducer phenotype CD45RA/T4 (p less than .01) were noted in the exposed group. Both kappa and lambda light-chain frequencies were elevated (p less than .01). Proliferative responses to the three mitogens tested, PHA, CONA, PWM, and to allogeneic lymphocytes in the mixed-lymphocyte culture assay were significantly lower than in controls (p less than .01). Responses in assays of the natural killer function were not significantly different from those of controls, but Fc receptor-associated K cell function was significantly greater than responses in controls. Of 12 individuals tested for evidence of autoimmunity, 11 demonstrated some increased titer of a form of autoantibody. This cluster of significant findings demonstrates the emergence of aberrant peripheral T and B cell regulation and a potential for autoimmune activation, detectable up to 10 y after exposure to technical chlordane.
对27名在家中或工作场所接触过工业氯丹的个体进行了淋巴细胞表型频率和体外功能测定研究。选择了一个由118名个体组成的对照组进行研究，这些个体在年龄和性别分布上与暴露组相似，且未曾有意识地接触过工业氯丹。在暴露组中，观察到循环中皮质胸腺细胞（CD1）频率显著增加（p小于0.001），而抑制诱导表型CD45RA/T4频率降低（p小于0.01）。κ和λ轻链频率均升高（p小于0.01）。在混合淋巴细胞培养试验中，对三种测试促有丝分裂原PHA、CONA、PWM以及对同种异体淋巴细胞的增殖反应显著低于对照组（p小于0.01）。自然杀伤功能测定中的反应与对照组无显著差异，但Fc受体相关K细胞功能显著高于对照组。在接受自身免疫证据检测的12名个体中，11名显示某种自身抗体滴度有所升高。这一系列显著发现表明，在接触工业氯丹长达10年后，外周T和B细胞调节出现异常，且存在自身免疫激活的可能性。
