Suppr超能文献

[牛源沙门氏菌分离株中肠毒素产生的检测]

[Detection of enterotoxin production in bovine Salmonella isolates].

作者信息

Dinjus U, Liebold R, Methner U, Selbitz H J, Steinbach G

机构信息

Institut für bakterielle Tierseuchenforschung Jena.

出版信息

Berl Munch Tierarztl Wochenschr. 1992 Jun 1;105(6):201-5.

PMID:1497581
Abstract

54 Salmonella-strains (7 serovars) of bovine origin isolated from faecal samples, rectal swabs as well as from organs of emergency-slaughtered or dead animals were tested for enterotoxin production (heat-labile and heat-stable) in rabbit-ileal-loop-assay (RILT), skin-permeability-factor-test (HPT), CHO- and Y1-cell-culture-assay and in baby-mouse-test (BMT). The cell-free supernatants (CFS) were used in the tests. The Y1-cells did not respond to the Salmonella toxins. While the RILT was suitable, the CHO-cell-assay proved to be the most sensitive and easy-to-handle system. The results confirm the frequent occurrence of the biological property of enterotoxin production within the species Salmonella enterica. Therefore, this property is in our opinion not useful as an epizootological marker for salmonellae.

摘要

从粪便样本、直肠拭子以及紧急屠宰或死亡动物的器官中分离出54株源自牛的沙门氏菌菌株(7个血清型),通过兔回肠袢试验(RILT)、皮肤通透性因子试验(HPT)、CHO细胞和Y1细胞培养试验以及幼鼠试验(BMT)检测其肠毒素产生情况(热不稳定和热稳定)。试验中使用了无细胞上清液(CFS)。Y1细胞对沙门氏菌毒素无反应。虽然RILT适用,但CHO细胞试验被证明是最敏感且易于操作的系统。结果证实了肠炎沙门氏菌物种内产生肠毒素生物学特性的频繁发生。因此,我们认为该特性作为沙门氏菌的动物流行病学标志物并无用处。

文献检索

告别复杂PubMed语法,用中文像聊天一样搜索,搜遍4000万医学文献。AI智能推荐,让科研检索更轻松。

立即免费搜索

文件翻译

保留排版,准确专业,支持PDF/Word/PPT等文件格式,支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述,25分钟生成高质量综述,智能提取关键信息,辅助科研写作。

立即免费体验