Dinjus U, Liebold R, Methner U, Selbitz H J, Steinbach G
Institut für bakterielle Tierseuchenforschung Jena.
Berl Munch Tierarztl Wochenschr. 1992 Jun 1;105(6):201-5.
54 Salmonella-strains (7 serovars) of bovine origin isolated from faecal samples, rectal swabs as well as from organs of emergency-slaughtered or dead animals were tested for enterotoxin production (heat-labile and heat-stable) in rabbit-ileal-loop-assay (RILT), skin-permeability-factor-test (HPT), CHO- and Y1-cell-culture-assay and in baby-mouse-test (BMT). The cell-free supernatants (CFS) were used in the tests. The Y1-cells did not respond to the Salmonella toxins. While the RILT was suitable, the CHO-cell-assay proved to be the most sensitive and easy-to-handle system. The results confirm the frequent occurrence of the biological property of enterotoxin production within the species Salmonella enterica. Therefore, this property is in our opinion not useful as an epizootological marker for salmonellae.
从粪便样本、直肠拭子以及紧急屠宰或死亡动物的器官中分离出54株源自牛的沙门氏菌菌株（7个血清型），通过兔回肠袢试验（RILT）、皮肤通透性因子试验（HPT）、CHO细胞和Y1细胞培养试验以及幼鼠试验（BMT）检测其肠毒素产生情况（热不稳定和热稳定）。试验中使用了无细胞上清液（CFS）。Y1细胞对沙门氏菌毒素无反应。虽然RILT适用，但CHO细胞试验被证明是最敏感且易于操作的系统。结果证实了肠炎沙门氏菌物种内产生肠毒素生物学特性的频繁发生。因此，我们认为该特性作为沙门氏菌的动物流行病学标志物并无用处。
