Vergara Daniela, Ávila Daniela, Escobar Elizabeth, Carrasco-Pozo Catalina, Sánchez Andrés, Gotteland Martin
Department of Nutrition, Faculty of Medicine, University of Chile, Santiago, Independencia 1027, Independencia, Santiago, Chile.
Nutr J. 2015 Mar 19;14:27. doi: 10.1186/s12937-015-0008-1.
Respiratory diseases are associated with pulmonary oxidative stress and inflammatory processes. Though studies in animal models suggest that dietary polyphenols improve lung injury, no intervention studies were carried out in humans. The aim of this study was to determine whether the intake of an anthocyanin-rich maqui extract improved H2O2 and IL-6 concentrations in exhaled breath condensates (EBCs) from asymptomatic smokers.
15 asymptomatic smokers with mild cigarette smoking (3 pack-year [2.4 - 7.7]) (mean [CI95%]) were recruited in this exploratory longitudinal study. They ingested 2 g of maqui extract (polyphenol content = 5.18 ± 2.00 g GAE/100 g; FRAP value = 27.1 ± 2.0 mmol Fe(++)/100 g), twice daily for two weeks. EBCs were collected before and after treatment and the changes in H2O2 and IL-6 concentrations were determined by fluorimetry and Elisa, respectively. The EBC contents of H2O2 and IL-6 H2O2 before and after treatment in smokers were also compared with those determined in single EBC samples from 8 healthy non-smokers subjects. At baseline, the H2O2 concentrations were higher and those of IL-6 lower in the smokers than in the non-smokers. Maqui extract significantly decreased H2O2 (p < 0.0002) and increased IL-6 (p < 0.004) in the EBC from smokers. The EBC concentrations of H2O2 and IL-6 after maqui administration did not differ between smokers and non-smokers.
Maqui extract normalizes IL-6 and H2O2 concentrations in EBC from humans with mild smoking habits. If confirmed, these results suggest that dietary polyphenols might be considered as an interesting alternative for the dietary management of respiratory disorders.
呼吸系统疾病与肺部氧化应激和炎症过程相关。尽管动物模型研究表明膳食多酚可改善肺损伤，但尚未在人类中开展干预研究。本研究旨在确定摄入富含花青素的智利酒果提取物是否能改善无症状吸烟者呼出气冷凝物（EBC）中的过氧化氢（H2O2）和白细胞介素-6（IL-6）浓度。
在这项探索性纵向研究中招募了15名轻度吸烟的无症状吸烟者（吸烟量为3包年[2.4 - 7.7]）（均值[95%置信区间]）。他们每天服用2克智利酒果提取物（多酚含量 = 5.18 ± 2.00克没食子酸当量/100克；铁还原抗氧化能力（FRAP）值 = 27.1 ± 2.0毫摩尔Fe(++)/100克），持续两周，每日两次。在治疗前后收集EBC，并分别通过荧光法和酶联免疫吸附测定法（ELISA）测定H2O2和IL-6浓度的变化。还将吸烟者治疗前后EBC中H2O2和IL-6的含量与8名健康非吸烟者单次EBC样本中测定的含量进行比较。在基线时，吸烟者中H2O2浓度高于非吸烟者，而IL-6浓度低于非吸烟者。智利酒果提取物显著降低了吸烟者EBC中的H2O2（p < 0.0002）并提高了IL-6（p < 0.004）。服用智利酒果提取物后，吸烟者和非吸烟者EBC中H2O2和IL-6的浓度没有差异。
智利酒果提取物可使轻度吸烟习惯人群EBC中的IL-6和H2O2浓度正常化。如果得到证实，这些结果表明膳食多酚可能被视为呼吸系统疾病饮食管理中一种有趣的替代方法。
