Neukirch Hannes, D'Ambrosio Michele, Dalla Via Josef, Guerriero Antonio
Laboratory of Bioorganic Chemistry, University of Trento, via Sommarive 14, 1-38050 Povo, Trento, Italy.
Phytochem Anal. 2004 Jan-Feb;15(1):30-5. doi: 10.1002/pca.739.
Dichloromethane extracts of dried flowers of Calendula officinalis contain eight known bioactive triterpendiol monoesters, namely, faradiol-3-O-palmitate, faradiol-3-O-myristate, faradiol-3-O-laurate, arnidiol-3-O-palmitate, arnidiol-3-O-myristate, arnidiol-3-O-laurate, calenduladiol-3-O-palmitate and calenduladiol-3-O-myristate. These pentacyclic terpenoids have been quantified simultaneously using reversed-phase HPLC with isocratic elution and internal standardisation. Of the 10 varieties of C. officinalis investigated, Calypso Orange Florensis produced the highest amounts of the bioactive monoesters, followed by Fiesta Gitana Gelb and May Orange Florensis. The lipophilic extract from the flowers of Calypso Orange Florensis variety also contained low levels of the newly characterised calenduladiol-3-O-laurate.
金盏花干燥花朵的二氯甲烷提取物含有八种已知的生物活性三萜二醇单酯，即法拉二醇-3-O-棕榈酸酯、法拉二醇-3-O-肉豆蔻酸酯、法拉二醇-3-O-月桂酸酯、阿诺二醇-3-O-棕榈酸酯、阿诺二醇-3-O-肉豆蔻酸酯、阿诺二醇-3-O-月桂酸酯、金盏二醇-3-O-棕榈酸酯和金盏二醇-3-O-肉豆蔻酸酯。这些五环萜类化合物已采用反相高效液相色谱法，通过等度洗脱和内标法同时进行了定量分析。在所研究的10个金盏花品种中，卡里普索橙弗洛伦西斯品种产生的生物活性单酯含量最高，其次是吉坦纳黄节曰和五月橙弗洛伦西斯。卡里普索橙弗洛伦西斯品种花朵的亲脂性提取物中还含有低水平的新鉴定出的金盏二醇-3-O-月桂酸酯。
