Butnariu Monica, Coradini Cristina Zepa
Chemistry and Biochemistry Department,Banat's University of Agricultural Sciences and Veterinary Medicine from Timisoara, Calea Aradului, no, 119, Timisoara, 300645 Romania.
Chem Cent J. 2012 Apr 27;6:35. doi: 10.1186/1752-153X-6-35.
This study aimed to quantify the active biological compounds in C. officinalis flowers. Based on the active principles and biological properties of marigolds flowers reported in the literature, we sought to obtain and characterize the molecular composition of extracts prepared using different solvents. The antioxidant capacities of extracts were assessed by using spectrophotometry to measure both absorbance of the colorimetric free radical scavenger 2,2-diphenyl-1-picrylhydrazyl (DPPH) as well as the total antioxidant potential, using the ferric reducing power (FRAP) assay.
Spectrophotometric assays in the ultraviolet-visible (UV-VIS) region enabled identification and characterization of the full range of phenolic and flavonoids acids, and high-performance liquid chromatography (HPLC) was used to identify and quantify phenolic compounds (depending on the method of extraction). Methanol ensured more efficient extraction of flavonoids than the other solvents tested.Antioxidant activity in methanolic extracts was correlated with the polyphenol content.
The UV-VIS spectra of assimilator pigments (e.g. chlorophylls), polyphenols and flavonoids extracted from the C. officinalis flowers consisted in quantitative evaluation of compounds which absorb to wavelengths broader than 360 nm.
本研究旨在对金盏花花中的活性生物化合物进行定量分析。基于文献中报道的万寿菊花的活性成分和生物学特性，我们试图获取并表征使用不同溶剂制备的提取物的分子组成。通过分光光度法测定比色自由基清除剂2,2-二苯基-1-苦基肼（DPPH）的吸光度以及使用铁还原能力（FRAP）测定法测定总抗氧化能力，来评估提取物的抗氧化能力。
紫外可见（UV-VIS）区域的分光光度法能够鉴定和表征酚酸和黄酮酸的全范围，并且使用高效液相色谱法（HPLC）来鉴定和定量酚类化合物（取决于提取方法）。甲醇比其他测试溶剂能更有效地提取黄酮类化合物。甲醇提取物中的抗氧化活性与多酚含量相关。
从金盏花花中提取的同化色素（如叶绿素）、多酚和黄酮类化合物的UV-VIS光谱在于对吸收波长大于360nm的化合物进行定量评估。
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