Zandbelt Michiel M, Vogelzangs Judith, Van De Putte Leo BA, Van Venrooij Walther J, Van Den Hoogen Frank HJ
Department of Rheumatology, University Medical Center St Radboud, Nijmegen, The Netherlands.
Arthritis Res Ther. 2004;6(1):R33-R38. doi: 10.1186/ar1021. Epub 2003 Oct 31.
The presence of anti-alpha-fodrin autoantibodies has been reported to be a highly specific and sensitive test for the diagnosis of Sjögren's syndrome (SjS). We looked (in Nijmegen) for anti-alpha-fodrin, anti-Ro60, and anti-La autoantibodies in a cohort of 51 patients with rheumatic diseases (primary SjS [21], secondary SjS 6, rheumatoid arthritis [RA] 12, systemic lupus erythematosus [SLE] 6, and scleroderma 6) and in 28 healthy subjects, using ELISA, immunoblotting, and immunoprecipitation. The same samples were analyzed with an alternative anti-alpha-fodrin ELISA in Hanover. The Nijmegen ELISA of the sera from primary SjS showed sensitivities of 43% and 48% for IgA- and IgG-type anti-alpha-fodrin antibodies, respectively. The Hanover ELISA showed sensitivities of 38% and 10% for IgA- and IgG-type anti-alpha-fodrin antibodies, respectively. The ELISAs for alpha-fodrin showed six (Nijmegen) and four (Hanover) anti-alpha-fodrin-positive RA sera. IgA and IgG anti-fodrin antibodies were also present in four patients with secondary SjS. The sensitivities of Ro60 and La-antibodies in the Nijmegen ELISA were 67% and 62%, respectively. Unlike anti-alpha-fodrin antibodies, all anti-Ro60 and anti-La positive sera could be confirmed by immunoblotting or RNA immunoprecipitation. Thus, anti-Ro and anti-La autoantibodies were more sensitive than anti-alpha-fodrin autoantibodies in ELISA and were more frequently confirmed by other techniques. Anti-La antibodies appear to be more disease-specific than anti-alpha-fodrin antibodies, which are also found in RA sera. Therefore, the measurement of anti-alpha-fodrin autoantibodies does not add much to the diagnosis of Sjögren's syndrome.
据报道，抗α- fodrin自身抗体的存在是诊断干燥综合征（SjS）的一项高度特异且敏感的检测方法。我们（在奈梅亨）采用酶联免疫吸附测定（ELISA）、免疫印迹法和免疫沉淀法，对51例风湿性疾病患者（原发性干燥综合征[21例]、继发性干燥综合征6例、类风湿关节炎[RA]12例、系统性红斑狼疮[SLE]6例和硬皮病6例）及28名健康受试者的抗α- fodrin、抗Ro60和抗La自身抗体进行了检测。在汉诺威，使用另一种抗α- fodrin ELISA对相同样本进行了分析。原发性干燥综合征患者血清的奈梅亨ELISA检测显示，IgA型和IgG型抗α- fodrin抗体的敏感性分别为43%和48%。汉诺威ELISA检测显示，IgA型和IgG型抗α- fodrin抗体的敏感性分别为38%和10%。α- fodrin的ELISA检测显示，有6例（奈梅亨）和4例（汉诺威）抗α- fodrin阳性的RA血清。4例继发性干燥综合征患者也存在IgA和IgG抗fodrin抗体。奈梅亨ELISA检测中Ro60和La抗体的敏感性分别为67%和62%。与抗α- fodrin抗体不同，所有抗Ro60和抗La阳性血清均可通过免疫印迹法或RNA免疫沉淀法得到证实。因此，在ELISA检测中，抗Ro和抗La自身抗体比抗α- fodrin自身抗体更敏感，且更常通过其他技术得到证实。抗La抗体似乎比抗α- fodrin抗体更具疾病特异性，抗α- fodrin抗体在RA血清中也可检测到。因此，抗α- fodrin自身抗体的检测对干燥综合征的诊断帮助不大。
