Yavuz Sule, Toker Ebru, Bicakcigil Muge, Mumcu Gonca, Cakir Sezen
Department of Rheumatology, and the Faculties of Health Education, University of Marmara Medical School, Istanbul, Turkey.
J Rheumatol. 2006 Jul;33(7):1289-92.
To evaluate levels of IgA and IgG antibodies against a-fodrin in serum, tear fluid, and saliva and compare them with anti-Ro and anti-La antibody levels in the same samples of patients with Sjögren's syndrome (SS).
Samples from 25 patients with SS (17 primary and 8 secondary), 8 patients with systemic lupus erythematosus (SLE), and 7 patients with rheumatoid arthritis (RA) as well as 20 healthy blood donor controls were collected. Antibodies were measured using ELISA.
Although 40% of patients with primary SS had IgG anti-a-fodrin in their sera, it was also found in 36% and 32% of samples of their tear fluid and saliva, respectively. IgA a-fodrin antibodies were detected in 32% of SS sera, 20% of tear fluid samples, and 32% of saliva samples. Although the level of IgG anti-a-fodrin was significantly greater in serum, tear fluid, and saliva of SS patients compared to controls (p < 0.001), a significant difference was observed only in serum and saliva. While anti-Ro was detected in 48%, 56%, and 24% of serum, tear fluid, and saliva samples, respectively, anti-La was found in 40%, 44%, and 28%. Significant association was observed between serum IgG antibodies against a-fodrin and dry eye symptom score and rose bengal staining score. A negative association was also noted between tear IgA antibodies against a-fodrin and Schirmer I test.
Correlation of IgG and IgA antibodies against a-fodrin with the severity of eye involvement suggests that these autoantibodies may be considered activation markers of SS.
评估血清、泪液和唾液中抗α - fodrin的IgA和IgG抗体水平，并将其与干燥综合征（SS）患者相同样本中的抗Ro和抗La抗体水平进行比较。
收集25例SS患者（17例原发性和8例继发性）、8例系统性红斑狼疮（SLE）患者、7例类风湿关节炎（RA）患者以及20名健康献血者对照的样本。使用酶联免疫吸附测定法（ELISA）检测抗体。
虽然40%的原发性SS患者血清中有IgG抗α - fodrin，但在其泪液样本和唾液样本中分别也有36%和32%检测到该抗体。在32%的SS血清、20%的泪液样本和32%的唾液样本中检测到IgA抗α - fodrin抗体。虽然与对照组相比，SS患者血清、泪液和唾液中的IgG抗α - fodrin水平显著更高（p < 0.001），但仅在血清和唾液中观察到显著差异。血清、泪液和唾液样本中抗Ro的检测率分别为48%、56%和24%，抗La的检测率分别为40%、44%和28%。血清中抗α - fodrin的IgG抗体与干眼症状评分和孟加拉玫瑰红染色评分之间存在显著关联。泪液中抗α - fodrin的IgA抗体与Schirmer I试验之间也存在负相关。
抗α - fodrin的IgG和IgA抗体与眼部受累严重程度的相关性表明，这些自身抗体可能被视为SS的激活标志物。
