Wang Sihong, Diller Kenneth R, Aggarwal Shanti J
Department of Biomedical Engineering, ENS612, 1 University Station C0800, University of Texas at Austin, Austin, TX 78712-0238, USA.
J Biomech Eng. 2003 Dec;125(6):794-7. doi: 10.1115/1.1632522.
The purpose of this study was to determine the kinetics of HSP70 expression in response to mild thermal stress. The rationale is to produce a basis for design of optimal heating methods to induce HSP70 expression for preconditioning in cardiac surgery. Bovine aortic endothelial cells were heated at 42 degrees C for 0.5 to 5 hours followed by 37 degrees C recovery for 1 to 48 hours. Quantitative analysis of western blot results showed HSP70 expression kinetics is a coupled function of heating temperature and time and of post-heating duration. Bimodal HSP70 expression kinetics were identified which may be an important cause of the "second window of protection" observed by other researchers.
本研究的目的是确定热休克蛋白70（HSP70）在轻度热应激反应中的表达动力学。其基本原理是为设计最佳加热方法提供依据，以诱导HSP70表达，用于心脏手术中的预处理。将牛主动脉内皮细胞在42℃加热0.5至5小时，然后在37℃恢复1至48小时。蛋白质印迹结果的定量分析表明，HSP70表达动力学是加热温度和时间以及加热后持续时间的耦合函数。确定了双峰HSP70表达动力学，这可能是其他研究人员观察到的“第二保护窗”的重要原因。
