Rylander Marissa Nichole, Diller Kenneth R, Wang Sihong, Aggarwal Shanti J
The University of Texas at Austin, Biomedical Engineering, 1 University Station C0800, Austin, TX 78712-0238, USA.
J Biomech Eng. 2005 Oct;127(5):751-7. doi: 10.1115/1.1993661.
Thermal preconditioning protocols for cardiac cells were identified which produce elevated HSP70 levels while maintaining high cell viability. Bovine aortic endothelial cells were heated with a water bath at temperatures ranging from 44 to 50 degrees C for periods of 1-30 min. Thermal stimulation protocols were determined which induce HSP70 expression levels ranging from 2.3 to 3.6 times the control while maintaining cell viabilities greater than 90%. An Arrhenius injury model fit to the cell damage data yielded values of A = 1.4 X 10(66) s(-1) and Ea = 4.1 X 10(5) J/mol. Knowledge of the injury parameters and HSP70 kinetics will enhance dosimetry guideline development for thermal stimulation of heat shock proteins expression in cardiac tissue.
已确定用于心脏细胞的热预处理方案，该方案在保持高细胞活力的同时可使HSP70水平升高。用44至50摄氏度的水浴对牛主动脉内皮细胞加热1至30分钟。确定了热刺激方案，该方案可诱导HSP70表达水平为对照的2.3至3.6倍，同时保持细胞活力大于90%。拟合细胞损伤数据的阿伦尼乌斯损伤模型得出A = 1.4×10(66) s(-1)和Ea = 4.1×10(5) J/mol的值。损伤参数和HSP70动力学知识将有助于制定心脏组织中热刺激热休克蛋白表达的剂量测定指南。
