Egutkin G G, Iakubovskiĭ S M, Gatsko G G
Ukr Biokhim Zh (1978). 1990 May-Jun;62(3):95-8.
The kinetic characteristics (Vmax and Km) of membrane-bound and serum 5'-nucleotidases from rats of different age groups are studied. The age-related increase of Km parameter in plasma membranes of fatty tissue and liver demonstrated a lower enzyme affinity to the substrate in ageing membranes. The specific activity of 5'-nucleotidase in blood serum of aged animals was below that in young ones, while Km value remained stable. Tissue differentiation of the catalytic properties of the enzyme and their aged-related characteristics may be to a considerable extent determined by the physicochemical state of cell membranes.
研究了不同年龄组大鼠膜结合型和血清5'-核苷酸酶的动力学特性（Vmax和Km）。脂肪组织和肝脏质膜中Km参数随年龄增长而增加，表明衰老膜中酶与底物的亲和力较低。老年动物血清中5'-核苷酸酶的比活性低于年轻动物，而Km值保持稳定。该酶催化特性的组织分化及其与年龄相关的特征在很大程度上可能由细胞膜的物理化学状态决定。
