Künneke Stephanie, Krüger Daniel, Janshoff Andreas
Johannes Gutenberg Universität Mainz, Institut für Physikalische Chemie, 55128 Mainz, Germany.
Biophys J. 2004 Mar;86(3):1545-53. doi: 10.1016/S0006-3495(04)74222-8.
A fast, quantitative, and unambiguous screening of material properties of biomembranes using scanning force microscopy in pulsed force mode on lipid membranes is presented. The spatially resolved study of breakthrough force, breakthrough distance, adhesion, stiffness, and topography of lipid membranes as determined simultaneously by digitalized pulsed force mode provides new insight into the structure-function relationship of model membranes, which are systematically analyzed by varying chain length, lipid headgroup, and lamellarity. For this purpose, a novel unbiased analysis method is presented. A strong correlation between adhesion and breakthrough events is found on lipid bilayers and multilayers and discussed in terms of structural stability and chemical and physical interactions. Our findings indicate that multilamellar 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoserine is mechanically strengthened with respect to material failure by calcium ions in solution.
本文介绍了一种在脂质膜上采用脉冲力模式的扫描力显微镜对生物膜材料特性进行快速、定量且明确的筛选方法。通过数字化脉冲力模式同时测定脂质膜的突破力、突破距离、粘附力、刚度和形貌的空间分辨研究，为模型膜的结构 - 功能关系提供了新的见解，通过改变链长、脂质头部基团和层状结构对其进行了系统分析。为此，提出了一种新颖的无偏分析方法。在脂质双层和多层膜上发现了粘附与突破事件之间的强相关性，并从结构稳定性以及化学和物理相互作用方面进行了讨论。我们的研究结果表明，多层的1 - 棕榈酰 - 2 - 油酰 - sn - 甘油 - 3 - 磷酸丝氨酸在溶液中因钙离子而在材料失效方面得到机械强化。
