Bolister N J, Johnson H E, Wathes C M
School of Veterinary Science, University of Bristol, Langford, United Kingdom.
Epidemiol Infect. 1992 Aug;109(1):121-31.
A strain of Klebsiella pneumoniae was aerosolized and its survival in air at different relative humidities was studied. Survival was dependent upon relative humidity and aerosols were most stable during storage at a relative humidity of 60%. Mice were exposed to aerosols of K. pneumoniae produced at this humidity and lung samples taken at timed intervals after exposure. Fifteen strains of K. pneumoniae were tested for their ability to colonize mice, but only five were detectable in mouse lungs 7 days after exposure. Three of these strains persisted without an increase in bacterial numbers, regardless of the initial inoculum used. Two strains of K. pneumoniae, designated strains 15 and 16, persisted in a similar manner when used at a low dose; however, when the dose received per lung was increased there was a rapid multiplication of bacteria in the lungs.
一株肺炎克雷伯菌被雾化，并研究了其在不同相对湿度空气中的存活情况。存活情况取决于相对湿度，气溶胶在相对湿度为60%的条件下储存时最为稳定。将小鼠暴露于在该湿度下产生的肺炎克雷伯菌气溶胶中，并在暴露后的不同时间间隔采集肺样本。对15株肺炎克雷伯菌进行了在小鼠体内定殖能力的测试，但暴露7天后，只有5株能在小鼠肺部检测到。其中3株持续存在，细菌数量没有增加，无论使用的初始接种量如何。两株肺炎克雷伯菌，即15号菌株和16号菌株，以低剂量使用时也以类似方式持续存在；然而，当每只肺接受的剂量增加时，肺部细菌会迅速繁殖。
