Gilmour M I, Wathes C M, Taylor F G
School of Veterinary Science, University of Bristol, Gt. Britain.
Vet Microbiol. 1990 Feb;21(4):363-75. doi: 10.1016/0378-1135(90)90008-j.
Pasteurella haemolytica A1 was aerosolised by a Collison nebuliser in a Henderson apparatus and its survival in air was measured. The organism was fragile in aerosol and survived best at high humidity and warm temperature. Mice were exposed to the aerosol and clearance from the lung measured. Deposition in the mouse lung showed a good linear correlation with bacterial concentration in the spray suspension fluid. Clearance from the lung was rapid over 24 h although some bacteria could be detected 2 and 4 days after exposure. Mice which received a second exposure 2 weeks later exhibited accelerated clearance from the lung whereby no bacteria could be detected after 12 h. This was associated with serum IgG antibody production, and local and splenic lymphocyte responses to bacterial antigen in vitro.
溶血巴斯德菌A1在亨德森装置中通过科里森雾化器雾化，并测定其在空气中的存活情况。该菌在气溶胶中较为脆弱，在高湿度和温暖温度下存活最佳。将小鼠暴露于气溶胶中，并测定肺部细菌清除情况。在小鼠肺部的沉积与喷雾悬浮液中的细菌浓度呈现良好的线性相关性。尽管在暴露后2天和4天仍可检测到一些细菌，但肺部细菌在24小时内快速清除。2周后接受第二次暴露的小鼠肺部细菌清除加速，12小时后未检测到细菌。这与血清IgG抗体产生以及体外对细菌抗原的局部和脾脏淋巴细胞反应有关。
