Iizawa Y, Nishi T, Kondo M, Imada A
Central Research Division, Takeda Chemical Industries, Ltd., Osaka.
Microbiol Immunol. 1988;32(9):895-906. doi: 10.1111/j.1348-0421.1988.tb01451.x.
Examination of mouse strain differences in susceptibility to experimental respiratory tract infection with Klebsiella pneumoniae 27 revealed that a chronic pulmonary infection model could be established using CBA/J mice. After 6 X 10(5) colony-forming units of K. pneumoniae 27 were inoculated into the lung, the bacterial counts in the lungs changed with time showing four different phases: initial decrease, regrowth, steady-state, and final increase leading to death. Throughout the course of the infection, the challenge bacteria were isolated mainly from the respiratory organs. Pulmonary gross lesions appeared on day 2 after infection and persisted thereafter. Lobar consolidation was the primary lesion and occurred mainly in the anterior and middle lobes of the right lung, and the median lobe. Mice began to die from 4 weeks after aerosol exposure. This model may be useful for investigating the pathogenesis of chronic pulmonary infection by Klebsiella and its therapy.
对小鼠品系对肺炎克雷伯菌27实验性呼吸道感染易感性差异的研究表明，使用CBA/J小鼠可建立慢性肺部感染模型。将6×10⁵个肺炎克雷伯菌27的菌落形成单位接种到肺部后，肺部细菌计数随时间变化呈现四个不同阶段：初期下降、再生长、稳态和最终导致死亡的增加。在整个感染过程中，攻击细菌主要从呼吸器官分离出来。感染后第2天出现肺部大体病变，并持续存在。大叶实变是主要病变，主要发生在右肺的前叶和中叶以及中叶。小鼠在气溶胶暴露后4周开始死亡。该模型可能有助于研究肺炎克雷伯菌慢性肺部感染的发病机制及其治疗方法。
