Park J-H, Park Y H, Seok S H, Cho S A, Kim D J, Lee H Y, Kim S H, Park J H
Departments of Laboratory Animal Medicine, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University, San 56-1, Shinlim-dong, Kwanak-ku, Seoul 151-742, South Korea.
J Comp Pathol. 2004 Feb-Apr;130(2-3):130-6. doi: 10.1016/j.jcpa.2003.10.001.
Suppurative gastritis was demonstrated in BALB/c mice 3 days after intragastric inoculation with 10(9) organisms of Listeria monocytogenes strain ATCC19113 (serotype 3). Also tested were four other strains of mice (C3H, C57BL/6, FVB and ICR) and three other strains of L. monocytogenes (HPB 3 [serotype 4b], HPB 410 [serotype 1/2a] and HPB 503 [serotype 1/2b]). After inoculation with ATCC19113 the numbers of bacteria found in the stomach wall were greater in C57BL/6 and ICR mice than in C3H and FVB mice; moreover, the gastritis produced in BALB/c and C57BL/6 mice was more severe than that produced in the other mouse strains. The gastritis produced in BALB/c mice with L. monocytogenes HPB 3, HPB 410 and HPB 503 was much more severe than that produced by ATCC19113. The inflammatory response occurred in the lamina muscularis and mucosa of the fundus. Massive necrosis of the gastric epithelium was observed, and there was oedema in a large part of the mucosal layer of the fundus. In addition, the submucosal layer was apparently expanded due to oedema, and in the cardia, the mucosal layer had become thin and flattened. Immunohistochemically, a polyclonal antibody against Listeria spp. produced labelling in areas of the gastric mucosa in which there was an inflammatory response and gastric epithelial necrosis.
在用10⁹个单核细胞增生李斯特菌菌株ATCC19113（血清型3）进行胃内接种后3天，在BALB/c小鼠中证实了化脓性胃炎。还对其他四种小鼠品系（C3H、C57BL/6、FVB和ICR）以及其他三种单核细胞增生李斯特菌菌株（HPB 3 [血清型4b]、HPB 410 [血清型1/2a]和HPB 503 [血清型1/2b]）进行了测试。接种ATCC19113后，C57BL/6和ICR小鼠胃壁中发现的细菌数量比C3H和FVB小鼠多；此外，BALB/c和C57BL/6小鼠产生的胃炎比其他小鼠品系更严重。用单核细胞增生李斯特菌HPB 3、HPB 410和HPB 503在BALB/c小鼠中产生的胃炎比ATCC19113产生的胃炎严重得多。炎症反应发生在胃底的肌层和黏膜。观察到胃上皮的大片坏死，胃底黏膜层的大部分出现水肿。此外，黏膜下层因水肿明显扩张，在贲门处，黏膜层变薄变平。免疫组织化学检测显示，针对李斯特菌属的多克隆抗体在胃黏膜有炎症反应和胃上皮坏死的区域产生标记。
