Barbour A H, Rampling A, Hormaeche C E
Department of Pathology, University of Adelaide, Australia.
Microb Pathog. 1996 Apr;20(4):247-53. doi: 10.1006/mpat.1996.0023.
The infectivity of 19 haemolytic isolates of Listeria monocytogenes from different sources (clinical and environmental) and representative isolates from Listeria ivanovii and Listeria innocua was compared following intragastric (i.g.) and intravenous (i.v.) inoculation in immunocompetent male BALB/c mice. There was marked variation in the infectivity of the different isolates by either route but when isolates were ranked in descending order by spleen count, following i.g. administration, the strains fell into four groups. Infectivity of some isolates also differed when i.v. inoculation was compared with i.g. administration, so that assessment of virulence by spleen counts only following i.v. inoculation might fail to detect isolates of poor infectivity by the i.g. route. These results suggest that intragastric inoculation of normal immunocompetent mice is a useful model for detecting strains of L. monocytogenes that are poorly invasive via the gut even though they are relatively virulent by intravenous inoculation.
在免疫功能正常的雄性BALB/c小鼠中，经胃内（i.g.）和静脉内（i.v.）接种后，比较了来自不同来源（临床和环境）的19株单核细胞增生李斯特菌溶血分离株以及伊氏李斯特菌和无害李斯特菌的代表性分离株的感染性。通过任一途径，不同分离株的感染性都有显著差异，但经胃内给药后，若按脾脏计数将分离株从高到低排序，这些菌株可分为四组。与胃内给药相比，静脉内接种时一些分离株的感染性也有所不同，因此仅通过静脉内接种后的脾脏计数来评估毒力，可能无法检测出经胃内途径感染性较差的分离株。这些结果表明，对正常免疫功能小鼠进行胃内接种是一种有用的模型，可用于检测经肠道侵袭性较差的单核细胞增生李斯特菌菌株，即使它们经静脉接种时毒力相对较强。
