Saleh I M, Ruyter I E, Haapasalo M, Ørstavik D
NIOM, Scandinavian Institute of Dental Materials, Haslum, Norway.
Int Endod J. 2004 Mar;37(3):193-8. doi: 10.1111/j.0143-2885.2004.00785.x.
To investigate the ability of different endodontic sealers and calcium hydroxide to kill bacteria in experimentally infected dentinal tubules.
Fifty-six human root segments were enlarged to size 2 (ISO size 090) Largo Peeso Reamer. After treatment with 17% EDTA and 5% NaOCl for 4 min each, the specimens were infected with Enterococcus faecalis for 3 weeks. The roots were divided into eight groups and filled with gutta-percha and AH Plus (AH); Grossman's sealer (GS); Ketac-Endo (KE); Apexit (AP); RoekoSeal Automix (RSA); or RoekoSeal Automix with an experimental primer (RP), or calcium hydroxide (CH) only. One group of specimens was left unfilled for control (CT). Following storage in humid conditions at 37 degrees C for 7 days, the root canals were re-established with new sterile Largo size 2. Dentine samples from each canal were then collected using a sterile size 5 (ISO size 150) Largo Peeso Reamer. The number of colony-forming units (CFU) was determined for each sample.
The mean log10 CFU in all test groups was significantly lower (P < 0.05) than that in the CT group. Root filling with AH and GS killed bacteria (mean CFU = 0) in the dentinal tubules. The mean log10 CFU for the CH group (0.53) was lower than that of RSA, AP, RP and KE (1.36, 1.40, 1.46 and 1.94, respectively), but only the difference between the CH and the KE groups was statistically significant (P < 0.05).
Root fillings in vitro with gutta-percha and AH or GS were effective in killing E. faecalis in dentinal tubules. Other endodontic sealers, as well as CH, were less effective.
研究不同根管封闭剂和氢氧化钙杀灭实验性感染牙本质小管内细菌的能力。
将56个人类牙根段扩大至2号（ISO 090号）拉戈皮索扩孔钻大小。分别用17%乙二胺四乙酸（EDTA）和5%次氯酸钠（NaOCl）处理4分钟后，将标本用粪肠球菌感染3周。将牙根分为八组，分别用牙胶尖和AH Plus（AH）、格罗斯曼封闭剂（GS）、Ketac-Endo（KE）、Apexit（AP）、RoekoSeal Automix（RSA）、或添加实验性底漆的RoekoSeal Automix（RP）填充，或仅用氢氧化钙（CH）填充。一组标本不做填充作为对照（CT）。在37℃潮湿条件下储存7天后，用新的无菌2号拉戈器械重新建立根管。然后用无菌5号（ISO 150号）拉戈皮索扩孔钻从每个根管收集牙本质样本。测定每个样本的菌落形成单位（CFU）数量。
所有测试组的平均log10 CFU均显著低于CT组（P < 0.05）。用AH和GS进行根管充填可杀灭牙本质小管内的细菌（平均CFU = 0）。CH组的平均log10 CFU（0.53）低于RSA、AP、RP和KE组（分别为1.36、1.40、1.46和1.94），但仅CH组和KE组之间的差异具有统计学意义（P < 0.05）。
体外使用牙胶尖和AH或GS进行根管充填可有效杀灭牙本质小管内的粪肠球菌。其他根管封闭剂以及CH的效果较差。
