Paillard S, Schnurbusch T, Tiwari R, Messmer M, Winzeler M, Keller B, Schachermayr G
Swiss Federal Research Station for Agroecology and Agriculture (FAL-Reckenholz), Reckenholzstrasse 191, 8046 Zurich, Switzerland.
Theor Appl Genet. 2004 Jul;109(2):323-32. doi: 10.1007/s00122-004-1628-6. Epub 2004 Mar 10.
Fusarium head blight (FHB) of wheat is a widespread and destructive disease which occurs in humid and semi-humid areas. FHB epidemics can cause serious yield and quality losses under favorable climatic conditions, but the major concern is the contamination of grains with mycotoxins. Resistance to FHB is quantitatively inherited and greatly influenced by the environment. Its evaluation is costly and time-consuming. The genetic basis of FHB resistance has mainly been studied in spring wheat. The objective of this study was to map quantitative trait loci (QTLs) for resistance to FHB in a population of 240 recombinant inbred lines (RILs) derived from a cross between the two Swiss winter wheat cultivars Arina (resistant) and Forno (susceptible). The RILs were genotyped with microsatellite and RFLP markers. The resulting genetic map comprises 380 loci and spans 3,086 cM. The 240 RILs were evaluated for resistance to FHB in six field trials over 3 years. Composite interval mapping (CIM) analyses carried out on FHB AUDPC (i.e. mean values across six environments) revealed eight QTLs which altogether explained 47% of the phenotypic variance. The three main QTLs were mapped on the long arms of chromosomes 6D ( R(2)=22%), 5B ( R(2)=14%) and 4A ( R(2)=10%). The QTL detected on 5B originated from the susceptible parent Forno. Other QTLs with smaller effects on FHB resistance were detected on chromosomes 2AL, 3AL, 3BL, 3DS and 5AL.
小麦赤霉病是一种广泛存在且具有破坏性的病害，发生于湿润和半湿润地区。在适宜的气候条件下，赤霉病流行会导致严重的产量和品质损失，但主要问题是谷物被霉菌毒素污染。对赤霉病的抗性是数量遗传的，且受环境影响很大。其评估成本高且耗时。赤霉病抗性的遗传基础主要在春小麦中进行了研究。本研究的目的是在由两个瑞士冬小麦品种阿丽娜（抗病）和福尔诺（感病）杂交产生的240个重组自交系（RIL）群体中定位抗赤霉病的数量性状位点（QTL）。利用微卫星和RFLP标记对RIL进行基因分型。构建的遗传图谱包含380个位点，跨度为3086厘摩。在3年的6次田间试验中对240个RIL的赤霉病抗性进行了评估。对赤霉病病情曲线下面积（AUDPC，即六个环境中的平均值）进行的复合区间作图（CIM）分析揭示了8个QTL，共解释了47%的表型变异。三个主要QTL分别位于6D染色体长臂（R² = 22%）、5B染色体长臂（R² = 14%）和4A染色体长臂（R² = 10%）上。在5B染色体上检测到的QTL来自感病亲本福尔诺。在2AL、3AL、3BL、3DS和5AL染色体上还检测到了对赤霉病抗性影响较小的其他QTL。
