小麦VRN2基因是一种受春化作用下调的开花抑制因子。
The wheat VRN2 gene is a flowering repressor down-regulated by vernalization.
作者信息
Yan Liuling, Loukoianov Artem, Blechl Ann, Tranquilli Gabriela, Ramakrishna Wusirika, SanMiguel Phillip, Bennetzen Jeffrey L, Echenique Viviana, Dubcovsky Jorge
机构信息
Department of Agronomy and Range Science, University of California, Davis, CA 95616, USA.
出版信息
Science. 2004 Mar 12;303(5664):1640-4. doi: 10.1126/science.1094305.
Abstract
Plants with a winter growth habit flower earlier when exposed for several weeks to cold temperatures, a process called vernalization. We report here the positional cloning of the wheat vernalization gene VRN2, a dominant repressor of flowering that is down-regulated by vernalization. Loss of function of VRN2, whether by natural mutations or deletions, resulted in spring lines, which do not require vernalization to flower. Reduction of the RNA level of VRN2 by RNA interference accelerated the flowering time of transgenic winter-wheat plants by more than a month.
摘要
具有冬季生长习性的植物在暴露于低温环境几周后会提前开花，这一过程称为春化作用。我们在此报告小麦春化基因VRN2的定位克隆，VRN2是开花的显性抑制因子，在春化作用下会被下调。VRN2功能缺失，无论是自然突变还是缺失，都会导致形成春性品系，这类品系开花不需要春化作用。通过RNA干扰降低VRN2的RNA水平，可使转基因冬小麦植株的开花时间提前一个多月。
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本文引用的文献
1
RFLP mapping of five major genes and eight quantitative trait loci controlling flowering time in a winter x spring barley (Hordeum vulgare L.) cross.控制冬春大麦（Hordeum vulgare L.）开花时间的五个主要基因和八个数量性状位点的 RFLP 图谱。
Genome. 1995 Jun;38(3):575-85. doi: 10.1139/g95-074.
2
Vernalization in Arabidopsis thaliana is mediated by the PHD finger protein VIN3.拟南芥中的春化作用由含植物同源结构域（PHD）的蛋白VIN3介导。
Nature. 2004 Jan 8;427(6970):159-64. doi: 10.1038/nature02195.
3
Analysis of the molecular basis of flowering time variation in Arabidopsis accessions.拟南芥种质资源开花时间变异的分子基础分析。
Plant Physiol. 2003 Jun;132(2):1107-14. doi: 10.1104/pp.103.021212. Epub 2003 May 22.
4
Positional cloning of the wheat vernalization gene VRN1.小麦春化基因VRN1的定位克隆
Proc Natl Acad Sci U S A. 2003 May 13;100(10):6263-8. doi: 10.1073/pnas.0937399100. Epub 2003 May 1.
5
The evolution of CONSTANS-like gene families in barley, rice, and Arabidopsis.大麦、水稻和拟南芥中CONSTANS类基因家族的进化
Plant Physiol. 2003 Apr;131(4):1855-67. doi: 10.1104/pp.102.016188.
6
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Science. 2002 Jul 12;297(5579):243-6. doi: 10.1126/science.1072147.
7
Functional importance of conserved domains in the flowering-time gene CONSTANS demonstrated by analysis of mutant alleles and transgenic plants.通过对突变等位基因和转基因植物的分析揭示开花时间基因CONSTANS中保守结构域的功能重要性。
Plant J. 2001 Dec;28(6):619-31. doi: 10.1046/j.1365-313x.2001.01163.x.
8
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9
Molecular analysis of FRIGIDA, a major determinant of natural variation in Arabidopsis flowering time.拟南芥开花时间自然变异的主要决定因素FRIGIDA的分子分析。
Science. 2000 Oct 13;290(5490):344-7. doi: 10.1126/science.290.5490.344.
10
Epistatic interaction between vernalization genes Vrn-Am1 and Vrn-Am2 in diploid wheat.二倍体小麦中春化基因Vrn-Am1和Vrn-Am2之间的上位性相互作用。
J Hered. 2000 Jul-Aug;91(4):304-6. doi: 10.1093/jhered/91.4.304.
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