Yan Liuling, Loukoianov Artem, Blechl Ann, Tranquilli Gabriela, Ramakrishna Wusirika, SanMiguel Phillip, Bennetzen Jeffrey L, Echenique Viviana, Dubcovsky Jorge
Department of Agronomy and Range Science, University of California, Davis, CA 95616, USA.
Science. 2004 Mar 12;303(5664):1640-4. doi: 10.1126/science.1094305.
Plants with a winter growth habit flower earlier when exposed for several weeks to cold temperatures, a process called vernalization. We report here the positional cloning of the wheat vernalization gene VRN2, a dominant repressor of flowering that is down-regulated by vernalization. Loss of function of VRN2, whether by natural mutations or deletions, resulted in spring lines, which do not require vernalization to flower. Reduction of the RNA level of VRN2 by RNA interference accelerated the flowering time of transgenic winter-wheat plants by more than a month.
具有冬季生长习性的植物在暴露于低温环境几周后会提前开花,这一过程称为春化作用。我们在此报告小麦春化基因VRN2的定位克隆,VRN2是开花的显性抑制因子,在春化作用下会被下调。VRN2功能缺失,无论是自然突变还是缺失,都会导致形成春性品系,这类品系开花不需要春化作用。通过RNA干扰降低VRN2的RNA水平,可使转基因冬小麦植株的开花时间提前一个多月。