Quek Chai-Hoon, Li Jun, Sun Tao, Chan Melinda Ling Hou, Mao Hai-Quan, Gan Leong Ming, Leong Kam W, Yu Hanry
Institute of Materials Research and Engineering (IMRE), 3 Research Link, Singapore 117602, Singapore.
Biomaterials. 2004 Aug;25(17):3531-40. doi: 10.1016/j.biomaterials.2003.09.112.
New anionic polyelectrolyte tetra-copolymers with photo-crosslinkable 4-(4-methoxycinnamoyl)phenyl methacrylate monomer in addition to a HEMA-MMA-MAA ter-copolymer system were synthesized. The tetra-copolymers were used to form photo-crosslinkable microcapsules with modified collagen by complex coacervation for rat hepatocytes encapsulation. The hepatocytes were encapsulated within a two-layered membrane comprising of modified collagen as the inner core and an outer photo-crosslinkable copolymer shell. Upon photo-crosslinking of the microcapsules with UV-Vis light irradiation, the mechanical strength and chemical stability of the microcapsules, and the cellular functions of the encapsulated hepatocytes were enhanced. Particularly, the mechanical stability of the microcapsules was dramatically strengthened. The new photo-crosslinkable tetra-copolymer formulation described in this article has opened a way to the development of hepatocyte microencapsulation technology for bioartifical liver assist device.
合成了新型阴离子型聚电解质四元共聚物，其除了含有甲基丙烯酸-2-羟乙酯-甲基丙烯酸甲酯-甲基丙烯酸三元共聚物体系外，还含有可光交联的4-(4-甲氧基肉桂酰基)苯基甲基丙烯酸酯单体。这些四元共聚物通过复凝聚作用与改性胶原蛋白形成可光交联的微胶囊，用于大鼠肝细胞的包封。肝细胞被包裹在由改性胶原蛋白作为内核和外部可光交联共聚物壳组成的双层膜内。在用紫外-可见光照射使微胶囊发生光交联后，微胶囊的机械强度和化学稳定性以及被包裹肝细胞的细胞功能均得到增强。特别是，微胶囊的机械稳定性显著提高。本文所述的新型可光交联四元共聚物配方为生物人工肝辅助装置的肝细胞微囊化技术的发展开辟了一条道路。
