RIKEN Advanced Science Institute, Wako-shi, Saitama, Japan.
Acta Biomater. 2010 Oct;6(10):4005-10. doi: 10.1016/j.actbio.2010.05.018. Epub 2010 May 23.
A novel visible light-crosslinkable porcine gelatin was prepared for gelation and micropatterning. The preparation employed a photo-oxidation-induced crosslinking mechanism. First, furfuryl groups were incorporated into the gelatin. Second, the modified gelatin was mixed in water with Rose Bengal, which is a visible light sensitizer. Irradiation by visible light solidified the aqueous solution. In addition, when the solution was cast on a plate, dried and photo-irradiated in the presence of a photomask a micropattern was formed that matched the micropattern on the photomask. The gelatin-immobilized regions enhanced cell adhesion. It was also confirmed that the gelatin incorporating furfuryl and Rose Bengal have no significant toxicity. The photo-crosslinkable gelatin was employed as a direct pulp capping material in the dental field. Considering these results, this system could be useful as a new type of visible light-induced crosslinkable biosealant.
一种新型的可见光交联猪明胶被制备用于胶凝和微图案化。该制备采用光氧化诱导交联机制。首先，将糠基引入明胶中。其次，将改性明胶与孟加拉玫瑰红（一种可见光敏化剂）混合在水中。可见光照射使水溶液固化。此外，当溶液浇铸在平板上，干燥并在光掩模存在下光照射时，形成与光掩模上的微图案匹配的微图案。固定明胶的区域增强了细胞黏附。还证实了含有糠基和孟加拉玫瑰红的明胶没有显著的毒性。光交联明胶被用作牙科领域的直接盖髓材料。考虑到这些结果，该系统可用作新型的可见光诱导交联生物密封剂。
