Schleper C, Kubo K, Zillig W
Max-Planck-Institut für Biochemie, Martinsried, Federal Republic of Germany.
Proc Natl Acad Sci U S A. 1992 Aug 15;89(16):7645-9. doi: 10.1073/pnas.89.16.7645.
The lemon-shaped "virus-like" particle SSV1 produced by the thermophilic archaeon Sulfolobus shibatae has not previously been observed to infect any host. Using a plaque assay suitable for the extreme growth conditions of this archaeon, we have shown infection of Sulfolobus solfataricus by SSV1. Upon infection, the viral genome was always found integrated into a tRNA gene of the host chromosome, a situation similar to that in S. shibatae, proving that site-specific integration is involved in establishing the lysogenic state. As in S. shibatae, UV-irradiation of lysogenized S. solfataricus led to virus production apparently not accompanied by cell lysis. We have also demonstrated the efficient uptake of exogenous DNA and its expression in Sulfolobus by transfecting S. solfataricus with SSV1 DNA by electroporation. Transfection efficiencies of up to 10(6) transfectants per microgram of DNA were obtained.
嗜热古菌柴田硫化叶菌产生的柠檬状“病毒样”颗粒SSV1此前未被观察到能感染任何宿主。通过适用于该古菌极端生长条件的噬菌斑测定法，我们证明了SSV1能感染嗜热栖热菌。感染后，总是发现病毒基因组整合到宿主染色体的一个tRNA基因中，这种情况与柴田硫化叶菌中的情况相似，证明位点特异性整合参与了溶原状态的建立。与柴田硫化叶菌一样，对溶源化的嗜热栖热菌进行紫外线照射会导致病毒产生，且显然不伴有细胞裂解。我们还通过电穿孔用SSV1 DNA转染嗜热栖热菌，证明了外源DNA在硫化叶菌中的有效摄取及其表达。每微克DNA获得了高达10⁶个转染子的转染效率。
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