Kreimer Aimee R, Alberg Anthony J, Viscidi Rapheal, Gillison Maura L
Department of Epidemiology, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland, USA.
Sex Transm Dis. 2004 Apr;31(4):247-56. doi: 10.1097/01.olq.0000118425.49522.2c.
The elevated risk for incident head and neck cancer among human papillomavirus (HPV)-16-seropositive individuals has substantiated a role for HPV in the etiology of head and neck cancers. The relationship between HPV seroreactivity and prevalent oral HPV infection in men and women without cancer has yet to be investigated.
The goal of this study was to evaluate a possible association between oral HPV infection and HPV seroreactivity after adjustment for gender, sexual behaviors, and sexually transmitted disease.
A cross-sectional study of factors associated with HPV-16, -18, and -33 seroreactivity was performed in a population of 586 men and women with and without HIV infection. Antibodies in sera were measured by use of a virus-like protein (VLP)-based enzyme-linked immunosorbent assay. Exfoliated cells from the tonsillar and oral mucosa were analyzed for the presence of 38 mucosal HPV types by polymerase chain reaction.
Women had significantly greater seroreactivity for all HPV types investigated when compared with men (odds ratio, 4.3; 95% confidence interval, 3.0-6.0). Seroprevalence was greatest in men and women aged 35 to 45 years. Tonsillar HPV infection, oral sex with men, and HIV infection were independently associated with HPV seroreactivity in men after adjustment for age and number of sexual partners. In women, HSV-2 seropositivity and a history of sexually transmitted diseases were similarly important. Oral and tonsillar HPV infection were not associated with HPV seroreactivity in women.
HPV seropositivity is associated with sexually transmitted diseases among women and possibly mucosal HPV exposures in men. Tonsillar HPV infection could impact seroprevalence, particularly in men.
人乳头瘤病毒（HPV）-16血清反应阳性个体发生头颈癌的风险升高，这证实了HPV在头颈癌病因学中的作用。HPV血清反应性与无癌症的男性和女性中普遍存在的口腔HPV感染之间的关系尚未得到研究。
本研究的目的是在调整性别、性行为和性传播疾病后，评估口腔HPV感染与HPV血清反应性之间可能存在的关联。
对586名有或无HIV感染的男性和女性人群进行了一项横断面研究，分析与HPV-16、-18和-33血清反应性相关的因素。通过基于病毒样蛋白（VLP）的酶联免疫吸附测定法检测血清中的抗体。通过聚合酶链反应分析扁桃体和口腔黏膜的脱落细胞中38种黏膜HPV类型的存在情况。
与男性相比，女性对所有研究的HPV类型的血清反应性显著更高（优势比，4.3；95%置信区间，3.0-6.0）。血清阳性率在35至45岁的男性和女性中最高。在调整年龄和性伴侣数量后，扁桃体HPV感染、与男性进行口交以及HIV感染与男性的HPV血清反应性独立相关。在女性中，HSV-2血清阳性和性传播疾病史同样重要。口腔和扁桃体HPV感染与女性的HPV血清反应性无关。
HPV血清阳性与女性的性传播疾病以及男性可能的黏膜HPV暴露有关。扁桃体HPV感染可能影响血清阳性率，尤其是在男性中。
