Vos A, Müller T, Cox J, Neubert L, Fooks A R
IDT GmbH, PO Box 214, 06855 Rosslau, Germany.
J Vet Med B Infect Dis Vet Public Health. 2004 Mar;51(2):55-60. doi: 10.1111/j.1439-0450.2004.00730.x.
Twenty ferrets (Mustela putorius furo) were inoculated by intramuscular (i.m.) injection with European Bat Lyssaviruses (EBLV) type-1 and 2 using 10(4.0) foci-forming units (FFU) EBLV-2 (n = 6), 10(4.0) FFU EBLV-1 (n = 7) and 10(6.0) FFU EBLV-1 (n = 7). Furthermore, 15 mice received 10(2.5) FFU EBLV-2 (n = 5), 10(2.5) FFU EBLV-1 (n = 5) and 10(4.5) FFU EBLV-1 (n = 5) by i.m. inoculation. All ferrets and mice receiving the higher dose of EBLV-1 succumbed to infection. In contrast, only three of seven ferrets and two of five mice inoculated experimentally with the lower EBLV-1 dose died. By comparison, all of the EBLV-2 infected ferrets and four of five mice survived infection. All 20 infected ferrets seroconverted. Using sensitive molecular tools, the virus was detected in different tissues, but it could not be found in any saliva samples taken during the 84-day observation period.
二十只雪貂（白鼬）通过肌肉注射接种了欧洲蝙蝠狂犬病病毒1型和2型，分别使用10⁴.⁰蚀斑形成单位（FFU）的EBLV - 2（n = 6）、10⁴.⁰ FFU的EBLV - 1（n = 7）和10⁶.⁰ FFU的EBLV - 1（n = 7）。此外，15只小鼠通过肌肉接种分别接受了10².⁵ FFU的EBLV - 2（n = 5）、10².⁵ FFU的EBLV - 1（n = 5）和10⁴.⁵ FFU的EBLV - 1（n = 5）。所有接受高剂量EBLV - 1的雪貂和小鼠均死于感染。相比之下，实验接种低剂量EBLV - 1的七只雪貂中只有三只死亡，五只小鼠中只有两只死亡。相比之下，所有感染EBLV - 2的雪貂和五只小鼠中的四只存活下来。所有20只感染的雪貂均出现血清转化。使用灵敏的分子工具，在不同组织中检测到了病毒，但在84天观察期内采集的任何唾液样本中均未发现病毒。
