Tjørnehøj K, Fooks A R, Agerholm J S, Rønsholt L
Department of Virology, Danish Institute for Food and Veterinary Research, Lindholm, DK-4771 Kalvehave, Denmark.
J Comp Pathol. 2006 Feb-Apr;134(2-3):190-201. doi: 10.1016/j.jcpa.2005.10.005. Epub 2006 Mar 20.
In 1998 and 2002, European bat lyssavirus type-1 (EBLV-1) was demonstrated in brain tissue of five Danish sheep suffering from neurological disorders. Four of the five sheep also had encephalic listeriosis. The animals originated from four flocks on pastures within a limited area of western Jutland. In a serological investigation in two of the herds, from which three of the diseased animals originated, EBLV-1 neutralizing antibodies were detected in only one of 69 sheep. In follow-up surveys, 2110 sheep sera collected at Danish slaughterhouses during 2000 were all negative for EBLV-1-antibodies, and EBLV-1 was not demonstrated in 87 ruminants displaying neurological symptoms. To investigate the pathogenic effects of EBLV-1, four sheep were inoculated intralabially with either brain material from one of the naturally infected sheep or virus isolated from the same sheep. These animals developed EBLV-1 neutralizing antibodies at 5-9 weeks post-inoculation but did not exhibit neurological signs during a 33-week observation period. It was speculated that the immune response prevented viral dissemination to the brain, resulting in an abortive peripheral infection. It was concluded that EBLV-1 can infect sheep under natural conditions as an incidental event.
1998年和2002年，在5只患有神经系统疾病的丹麦绵羊的脑组织中检测到1型欧洲蝙蝠狂犬病病毒（EBLV-1）。这5只绵羊中有4只还患有脑型李斯特菌病。这些动物来自日德兰半岛西部有限区域内四个牧场的四个羊群。在对其中两个羊群（三只患病动物来自这两个羊群）进行的血清学调查中，69只绵羊中只有1只检测到EBLV-1中和抗体。在后续调查中，2000年在丹麦屠宰场采集的2110份绵羊血清EBLV-1抗体均为阴性，并且在87只出现神经症状的反刍动物中未检测到EBLV-1。为了研究EBLV-1的致病作用，给4只绵羊经唇内接种了来自一只自然感染绵羊的脑材料或从同一只绵羊分离出的病毒。这些动物在接种后5-9周产生了EBLV-1中和抗体，但在33周的观察期内未表现出神经症状。据推测，免疫反应阻止了病毒传播到大脑，导致了一次流产性的外周感染。得出的结论是，EBLV-1在自然条件下可偶然感染绵羊。
