Zhang Hui, Yan Wei, Aebersold Ruedi
Institute for Systems Biology, 1441 N 34th Street, Seattle, Washington 98103-8904, USA.
Curr Opin Chem Biol. 2004 Feb;8(1):66-75. doi: 10.1016/j.cbpa.2003.12.001.
Quantitative proteome profiling using mass spectrometry and stable isotope dilution is being widely applied for the functional analysis of biological systems and for the detection of clinical, diagnostic or prognostic marker proteins. Because of the enormous complexity of proteomes, their comprehensive analysis is unlikely to be routinely achieved in the near future. However, in recent years, significant progress has been achieved focusing quantitative proteomic analyses on specific protein classes or subproteomes that are rich in biologically or clinically important information. Such projects typically combine the use of chemical probes that are specific for a targeted group of proteins and may contain stable isotope signatures for accurate quantification with automated tandem mass spectrometry and bioinformatics tools for data analysis. In this review, we summarize technical and conceptual advances in quantitative subproteome profiling based on tandem mass spectrometry and chemical probes.
使用质谱和稳定同位素稀释进行定量蛋白质组分析，正广泛应用于生物系统的功能分析以及临床、诊断或预后标志物蛋白的检测。由于蛋白质组极其复杂，近期内不太可能常规地实现对其全面分析。然而，近年来，在将定量蛋白质组分析聚焦于富含生物学或临床重要信息的特定蛋白质类别或亚蛋白质组方面，已取得显著进展。此类项目通常结合使用对目标蛋白质组具有特异性的化学探针，这些探针可能含有稳定同位素标记以便准确定量，同时结合自动串联质谱和用于数据分析的生物信息学工具。在本综述中，我们总结了基于串联质谱和化学探针的定量亚蛋白质组分析的技术和概念进展。
