Di Paola R, Marzocco S, Mazzon E, Dattola F, Rotondo F, Britti D, De Majo M, Genovese T, Cuzzocrea S
Institute of Pharmacology, School of Medicine, University of Messina, Torre Biologica, Policlinico Universitario, Via C. Valeria, Gazzi, 98100 Messina, Italy.
J Dent Res. 2004 Apr;83(4):343-8. doi: 10.1177/154405910408300414.
The role of nitric oxide and reactive oxygen species is well-demonstrated in inflammation. In this study, we evaluated the effect of aminoguanidine, a nitric oxide synthase inhibitor, in a rat model of periodontitis. We induced periodontitis in rats by placing a piece of 2/0 braided silk around the lower left 1st molar. At day 8, the gingivomucosal tissue encircling the mandibular 1st molar was removed for biochemical and histological analysis. Ligation significantly increased inducible nitric oxide synthase activity and expression, and damaged tissue revealed increased neutrophil infiltration, lipid peroxidation, and positive staining for nitrotyrosine formation and poly (ADP-ribose) polymerase activation. Ligation significantly increased Evans blue extravasation in gingivomucosal tissue and alveolar bone destruction. Aminoguanidine (100 mg/kg i.p., daily for 8 days) treatment significantly reduced all these inflammatory parameters, indicating that it protects against the tissue damage associated with periodontitis by reducing nitric oxide production and oxidative stress.
一氧化氮和活性氧在炎症中的作用已得到充分证实。在本研究中，我们评估了一氧化氮合酶抑制剂氨基胍在大鼠牙周炎模型中的作用。我们通过在大鼠左下第一磨牙周围放置一段2/0编织丝线来诱导牙周炎。在第8天，取下环绕下颌第一磨牙的龈黏膜组织进行生化和组织学分析。结扎显著增加了诱导型一氧化氮合酶的活性和表达，受损组织显示中性粒细胞浸润增加、脂质过氧化以及硝基酪氨酸形成和聚（ADP-核糖）聚合酶激活的阳性染色。结扎显著增加了龈黏膜组织中伊文思蓝渗出和牙槽骨破坏。氨基胍（100 mg/kg腹腔注射，每日一次，共8天）治疗显著降低了所有这些炎症参数，表明它通过减少一氧化氮生成和氧化应激来保护免受与牙周炎相关的组织损伤。
